We narrowed to 3,821 results for: SPL;

1. pMiNGo11-3

   Plasmid

   #126058

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   mitochondria Rieske iron sulphur protein (1-100 aa)
   ccdB cassette (BsaI)
   GFP11x3 (5 aa linker)
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMiNGo11-7

   Plasmid

   #126057

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   mitochondria Rieske iron sulphur protein (1-100 aa)
   ccdB cassette (BsaI)
   GFP11x7 (5 aa linker)
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST-SF3B2 R508K aa 401-550 fragment

   Plasmid

   #79674

   Purpose

   bacterial expression of GST-SF3B2 fragment (401-550) with R508L methylation site mutation

   Depositor

   Steven Clarke

   Article

   Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

   Insert

   splicing factor 3b subunit 2 (SF3B2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 451-550 with R508K

   Promoter

   tac

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

4. Lenti-Nova1-GFP

   Plasmid

   #217033

   Purpose

   Overexpression of Nova in DRG neurons promotes the "mature" splicing pattern observed in CNS neurons

   Depositor

   Chaolin Zhang

   Article

   Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

   Insert

   Nova1 (Nova1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMVTO-RBDK65E-R89L-nTEVp

   Plasmid

   #179670

   Purpose

   Mutant Ras Binding Domain split-nTEVP

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   CRaf RBD protein (RAF1 Human)
   

   Use

   Tags

   nTEVP

   Expression

   Mammalian

   Mutation

   K65E, R89L

   Promoter

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMVTO-RBDK65E-R89L-cTEVp

   Plasmid

   #179671

   Purpose

   Mutant Ras Binding Domain split-cTEVP

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   CRaf RBD protein (RAF1 Human)
   

   Use

   Tags

   cTEVP

   Expression

   Mammalian

   Mutation

   K65E, R89L

   Promoter

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHBS1506 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-L]

   Plasmid

   #133330

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All FYW to L in CTD of full-length TDP43

   Promoter

   Available since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHBS1507 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-S]

   Plasmid

   #133331

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All VLIM to S in CTD of full-length TDP43

   Promoter

   Available since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHBS1508 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-F]

   Plasmid

   #133332

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All VLIM to F in CTD of full-length TDP43

   Promoter

   Available since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHBS1397 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 W385G]

    Plasmid

    #118812

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W385G in CTD of full-length TDP43

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHBS1399 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W+G385W]

    Plasmid

    #118813

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G368W and W385G in CTD of full-length TDP43

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHBS1400 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G295F]

    Plasmid

    #118814

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G295F in CTD of full-length TDP43

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHBS1393 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaRRM]

    Plasmid

    #118805

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of RRM in full-length TDP43

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHBS1394 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaCTD]

    Plasmid

    #118806

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of CTD in full-length TDP43

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHBS1392 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaNTD]

    Plasmid

    #118807

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of NTD in full-length TDP43

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHBS1401 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]

    Plasmid

    #118808

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All F to S in CTD of full-length TDP43

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHBS1404 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-S]

    Plasmid

    #118809

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All VLIM to S in CTD of full-length TDP43

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHBS1405 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-F]

    Plasmid

    #118810

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All VLIM to F in CTD of full-length TDP43

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNeae2

    Plasmid

    #168300

    Purpose

    Bacterial surface display of polypeptides (nanobodies) in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:

    Insert

    Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
    

    Use

    Tags

    6xHis, E-tag, and myc-tag

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP

    Plasmid

    #90008

    Purpose

    Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    NBirA*-linker-FKBP
    FRB-linker-CBirA*
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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