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Plasmid#99378PurposeExpresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.DepositorInserthNGN2-P2A-Neo (NEUROG2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTetOAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL 4K>R
Plasmid#202420PurposeExpression of ubiquitination-deficient GFP-tagged PODXL (4 cytoplasmic lysines mutated to arginine)DepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationK431/525/526/547RPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL delta EC
Plasmid#202423PurposeExpression of GFP-tagged PODXL without extracellular domainDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationdeleted extracellular domainPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL 5N>Q
Plasmid#202419PurposeExpression of GFP-tagged PODXL with mutated N-linked glycosylation sitesDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationN33/43/104/144/360QPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBiex1-SYNAC
Plasmid#82710PurposeSynthetic soluble adenylyl cyclase FRET sensor and cAMP generatorDepositorUseTagsFLAG, mCerulean, and mCitrineExpressionBacterial and InsectMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL delta IC
Plasmid#202422PurposeExpression of GFP-tagged PODXL without intracellular domainDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationdeleted intracellular domainPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLuc-Optop38i-dsm
Plasmid#89748PurposeExpression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13FDepositorInsertOptop38i dark-state mutant
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationC450A mutation of AsLov2JalphaPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-GNL
Plasmid#188232PurposePolycistronic human Glis1, LIN28 and NANOG were cloned into the pMXs vectorDepositorUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmEos2-N1 GBP (GBP-mEos2)
Plasmid#162876PurposeExpression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization MicroscopyDepositorInsertGFP Binding Protein tagged with mEos2
UseTagsmEos2ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL delta PBM + FBM
Plasmid#202416PurposeExpression of GFP-tagged PODXL PDZ-binding and FERM-binding motif mutationDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationLacking DTHL, and H477/R479/S481>AAA mutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL FKBP 4K>R
Plasmid#202421PurposeExpression of ubiquitination-deficient GFP-tagged PODXL with FKBP-rapamycin binding (FRB) dimerization domainDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsFKBP and GFPExpressionMutationK431/525/526/547RPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL delta PBM
Plasmid#202415PurposeExpression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2)DepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationLacking DTHLPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL FBM
Plasmid#202417PurposeExpression of GFP-tagged PODXL FERM-binding motif mtuation (FBM; doesn't bind Ezrin)DepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationH477/R479/S481>AAA mutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL delta PBM + Ezrin
Plasmid#202418PurposeExpression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2) fused to EZRINDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsEzrin and GFPExpressionMutationdelete PDZ binding motifPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX303 TagRFP-T LGALS3 R186S
Plasmid#202428PurposeExpression of TagRFP-T-tagged Galectin 3 with mutation of carbohdrate recognition binding domain (CRD) (R186S mutant)DepositorInsertLGALS3 (LGALS3 Human)
UseLentiviralTagsTagRFPExpressionMutationR186SPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSP12::mCherry
Plasmid#30169DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagsmCherryExpressionMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTEC21
Plasmid#30180DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagsKaedeExpressionMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro
Plasmid#141395PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInserteGFP
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHN922
Plasmid#178975PurposeExpression of GST-His8-GFP-nanobodyDepositorInsertGST-His8-GFP-nanobody
UseAffinity Reagent/ AntibodyTagsGST, His8ExpressionBacterialMutationPromotertacAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
hIgG1-F1-Fc
Plasmid#194597PurposeMammalian expression of F1 nanobody upstream of human IgG1; F1 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertF1-Fc (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only