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pYSD005

Plasmid

#212902

Purpose

Encodes S. cerevisiae KSH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Publication

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

KSH1 (KSH1 Budding Yeast)

Expression

Bacterial

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMC8b-XI5up

Plasmid

#176172

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-5

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XI-5

Use

Synthetic Biology

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-XI5dw

Plasmid

#176173

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-5

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XI-5

Use

Synthetic Biology

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC8b-XII5up

Plasmid

#176176

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-5

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XII-5

Use

Synthetic Biology

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-XII5dw

Plasmid

#176177

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-5

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XII-5

Use

Synthetic Biology

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-X2

Plasmid

#176152

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-2

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-X3

Plasmid

#176153

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-3

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XI1

Plasmid

#176155

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-1

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XI5

Plasmid

#176158

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-5

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XII2

Plasmid

#176160

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-2

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC8b-X2up

Plasmid

#176162

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-2

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site X-2

Use

Synthetic Biology

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-X2dw

Plasmid

#176163

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-2

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site X-2

Use

Synthetic Biology

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pC0.122

Plasmid

#119629

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA scaffold

Use

Synthetic Biology

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.029

Plasmid

#119626

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

AbREry

Use

Synthetic Biology

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.120

Plasmid

#119616

Purpose

Level 0 part. Linker

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

AbR Down Linker

Use

Synthetic Biology

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.082

Plasmid

#119611

Purpose

Level 0 part. Terminator

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

TrrnB

Use

Synthetic Biology

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.078

Plasmid

#119609

Purpose

Level 0 part. Terminator

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Tcpc_operon

Use

Synthetic Biology

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.203

Plasmid

#119604

Purpose

Level 0 part. TSS promoter

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

J23103_TSS

Use

Synthetic Biology

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.083

Plasmid

#119592

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

J23119MH_V01

Use

Synthetic Biology

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.054

Plasmid

#119572

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

PpetE

Use

Synthetic Biology

Mutation

RBS* added (22 bp) upstream of ATG. Deletion of n…

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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