We narrowed to 11,803 results for: nsf

1. pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

   Plasmid

   #184632

   Purpose

   Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Gad67 (Gad1 Mouse)
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-pTH-iCre:EGFP-WPREpA

   Plasmid

   #213142

   Purpose

   Truncated rat TH promoter expressing iCre fused to EGFP

   Depositor

   Ulrik Gether

   Article

   Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

   Insert

   iCre
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   Truncated TH promoter from rat (Rattus norvegicus…

   Available Since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-M13-TevC-IRES-SP6-Cre

   Plasmid

   #171620

   Purpose

   M13-TevC with Cre

   Depositor

   Hyungbae Kwon

   Article

   Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

   Insert

   M13-TevC-IRES-SP6-Cre
   

   Use

   AAV and Synthetic Biology

   Tags

   6x His

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_hSyn1-SIO-stGtACR1-FusionRed

   Plasmid

   #105678

   Purpose

   Expresses a soma-targeted GtACR1 in Cre-expressing neurons

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

   Has Service

   AAV5

   Insert

   stGtACR1
   

   Use

   AAV

   Tags

   FusionRed and Kv2.1

   Expression

   Mammalian

   Promoter

   hSyn1

   Available Since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-PTRE-tight-hM3Dq-mCherry

   Plasmid

   #66795

   Purpose

   Expresses the DREADD receptor hM3Dq-mCherry under the control of tet-inducible promoter for use in TetTaging

   Depositor

   William Wisden

   Article

   Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

   Insert

   pTIGHT promoter-hM3Dq-mCherry
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   pTIGHT TRE

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-hSyn-SpdCas9-W3SL

   Plasmid

   #210708

   Purpose

   This Plasmid express hSyn promoter driven SpdCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   S. Pyogenes dCas9
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   hSyn

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-Flag-NPM1c

   Plasmid

   #131813

   Purpose

   Expresses Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   This is the cytoplasmic mutant causing AML, where…

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGA2ox2:GUS

   Plasmid

   #108564

   Purpose

   Arabidopsis transformation, expresses GA2ox2 promoter/GUS fusion to study expression pattern in roots

   Depositor

   Ton Bisseling

   Article

   Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

   Insert

   GA2ox2 promoter (GA2OX2 Mustard Weed)
   

   Tags

   GUS

   Expression

   Plant

   Promoter

   GA2ox2

   Available Since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pE-35S-miniSdd7-nCas9-PBE-GmPPO2

   Plasmid

   #204855

   Purpose

   For cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd7-nSpCas9(D10A)-UGI-mScarlet
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A in SpCas9

   Promoter

   35S, AtU6-26p

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLY005SB_pAAV-U6sg(BbsI)-EFS-SB100X

    Plasmid

    #192148

    Purpose

    T cell CRISPR AAV vector

    Depositor

    Sidi Chen

    Article

    Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. MTRAP-bio

    Plasmid

    #47746

    Purpose

    Expresses enzymatically monobiotinylated full-length MTRAP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MTRAP
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-CAG-SPOTlight-U2GCR

    Plasmid

    #164819

    Purpose

    Reporter to measure ISR state-dependent protein synthesis

    Depositor

    Nicole Calakos

    Article

    Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

    Insert

    SPOTlight-U2GCR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. EBA140-bio

    Plasmid

    #47742

    Purpose

    Expresses enzymatically monobiotinylated full-length EBA140 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised EBA140
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. GAMA-bio

    Plasmid

    #47747

    Purpose

    Expresses enzymatically monobiotinylated full-length GAMA ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised GAMA
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. PTEX150-bio

    Plasmid

    #47788

    Purpose

    Expresses enzymatically monobiotinylated full-length PTEX150 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised PTEX150
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET24a-ncOGT-FL

    Plasmid

    #190821

    Purpose

    For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E.coli

    Promoter

    T7

    Available Since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. RH1-bio

    Plasmid

    #47748

    Purpose

    Expresses enzymatically monobiotinylated partial RH1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RH1 (partial)
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDmAct5C::Cas9-2A-Neo

    Plasmid

    #176679

    Purpose

    Expression of Drosophila codon optimized spCas9 under Drosophila Act5C promoter

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    

    Use

    Crispr

    Expression

    Insect

    Mutation

    Drosophila codon optimized

    Promoter

    D. melanogaster Act5C

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. scADGFP

    Plasmid

    #114434

    Purpose

    Epilepsy promoter enhanced with Arc elements driving GFP with SV40E polyA

    Depositor

    Edward Perez-Reyes

    Article

    Burke et al Int J Mol Sci. 2023 Sep 23;24(19):14467.

    Insert

    Green fluorescent protein
    

    Use

    AAV

    Tags

    ER export sequence FCYENEV

    Expression

    Mammalian

    Promoter

    Novel synthetic activity-dependent promoter

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-N-Solo-mScarlet-TUBA1B

    Plasmid

    #207764

    Purpose

    Donor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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