We narrowed to 9,895 results for: EPO

1. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR

   Plasmid

   #207135

   Purpose

   DNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-in

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXPR_220

   Plasmid

   #164560

   Purpose

   Lentiviral vector that encodes two sgRNA expression cassettes (one constitutive, one inducible via Flp). Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

   Inserts

   First sgRNA cassette
   Second sgRNA cassette
   

   Use

   CRISPR and Lentiviral; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and Mouse U6

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV Flag-LRRK2 K17A/K18A/R1441G

   Plasmid

   #186013

   Purpose

   Flag-LRRK2 K17A/K18A/R1441G

   Depositor

   Suzanne Pfeffer

   Article

   Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

   Insert

   Flag-LRRK2 K17A/K18A/R1441G (LRRK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See depositor's comments below.

   Promoter

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBT9-FHIP

   Plasmid

   #198527

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-FHIP fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   FHIP (FHIP1B Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   silent mutations in codons 353 and 651 as well as…

   Promoter

   ADH1

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-GG-hUbC-EBPF2-gRNA-RBMS3

   Plasmid

   #185557

   Purpose

   Lentiviral backbone with EBFP2 reporter and gRNA targeting RBMS3

   Depositor

   Andrew Wilson

   Article

   Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

   Insert

   RBMS3 gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-GG-hUbC-EBPF2-gRNA-HHIP

   Plasmid

   #185551

   Purpose

   Lentiviral backbone with EBFP2 reporter and gRNA targeting HHIP

   Depositor

   Andrew Wilson

   Article

   Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

   Insert

   HHIP gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-GG-hUbC-EBPF2-gRNA-ADGRG6

   Plasmid

   #185554

   Purpose

   Lentiviral backbone with EBFP2 reporter and gRNA targeting ADGRG6

   Depositor

   Andrew Wilson

   Article

   Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

   Insert

   ADGRG6 gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIFEX-A2aGH

   Plasmid

   #160542

   Purpose

   pITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N/A

   Promoter

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEFEX1-A2aGH

   Plasmid

   #160543

   Purpose

   pYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N/A

   Promoter

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEFEX2-A2aGH

    Plasmid

    #160544

    Purpose

    pYES2 derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEFEX3-A2aGH

    Plasmid

    #160545

    Purpose

    pYES2 derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFEX1-A2aGH

    Plasmid

    #160546

    Purpose

    pYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCFEX2-A2aGH

    Plasmid

    #160547

    Purpose

    pYC2/CT derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCFEX3-A2aGH

    Plasmid

    #160548

    Purpose

    pYC2/CT derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMH011_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid

    #179434

    Purpose

    Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-HS4-mch (SH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL006_phiC31_neo-5xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry

    Plasmid

    #179425

    Purpose

    Dual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-(nospacer)-mch
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. psiCheck Cdk2ap1ΔN (MT2B2)

    Plasmid

    #178029

    Purpose

    expression vector to test translation efficiency by luciferase reporter.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Luciferase; Nonviral

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Cloned in

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmirGlo-RBM8A-3'UTR 240-546

    Plasmid

    #158108

    Purpose

    reporter 3'UTR hRBM8A (short)

    Depositor

    Emanuela Felley-Bosco

    Article

    Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

    Insert

    3'UTR of hRBM8A (RBM8A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. SIN40C.TRE.mArid3a.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

    Plasmid

    #169318

    Purpose

    Doxycycline-inducible expression of murine Arid3a cDNA with dTomato as reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    Arid3a (Arid3a Mouse)
    

    Use

    Lentiviral

    Tags

    IRES-dTomato

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOTTC1535 pscAAV mU6 shRNA(scram) CMV-IE Nuc-EYFP

    Plasmid

    #135563

    Purpose

    An AAV vector expressing scrambled shRNA and a nuclear EYFP reporter

    Depositor

    Christopher Richie

    Article

    Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

    Inserts

    shRNA (scrambled)
    Nuc-EYFP
    

    Use

    AAV and RNAi

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE and mU6

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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