We narrowed to 16,586 results for: EMB

1. pRDB_118

   Plasmid

   #216089

   Purpose

   Cas9 [Sp] CRISPRa targeting CD47, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD47 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AA050

   Plasmid

   #216059

   Purpose

   Fragmid fragment: (2A Selection) violet-excited GFP

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   P2A_v1.1; VexGFP_v1.1; Stop
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRDB_161

   Plasmid

   #216091

   Purpose

   Cas9 [Sp] CRISPRi targeting CD81, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD81 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRDB_166

   Plasmid

   #216093

   Purpose

   Cas9 [Sp] CRISPRi targeting CD55, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD55 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AA076

   Plasmid

   #216021

   Purpose

   Fragmid fragment: (Cas protein) Cas13d

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas13d_v1.1 [Rfx]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AA012

   Plasmid

   #215974

   Purpose

   Fragmid fragment: (N' terminus) Bipartite Nuclear Localization Signal

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Start; BPNLS_v1.1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AA370

   Plasmid

   #215988

   Purpose

   Fragmid fragment: (N' terminus) attenuate expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Start; u(S)ORF_v1.1; Stop; Start; BPNLS_v1.1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRDA_833

   Plasmid

   #216086

   Purpose

   Cas9 [Sp] knockout targeting CD47, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD47 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-4T1-GST-TEV-NAP1

   Plasmid

   #217610

   Purpose

   For expression in E. coli of NAP1

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   TAC promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac-Dual-GST-TEV-SINTBAD-mCherry

    Plasmid

    #208874

    Purpose

    For expression in insect cells of SINTBAD-mCherry

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP

    Plasmid

    #179717

    Purpose

    Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lyn11-BirA*-HA

    Plasmid

    #214474

    Purpose

    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.

    Depositor

    Natalie Ahn

    Article

    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

    Insert

    BirA(R118G)
    

    Use

    Lentiviral; Gateway

    Tags

    HA tag and Lyn11 (Plasma Membrane Localization Se…

    Expression

    Mammalian

    Mutation

    R118G

    Promoter

    EF-1a

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET SUMO-syt1 (96-421)

    Plasmid

    #213619

    Purpose

    Bacterial expression of SUMO-tagged rat syt1 (96-421)

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt1 (96-421) (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET SUMO-syt1 (96-421) I367C

    Plasmid

    #213620

    Purpose

    Bacterial expression of SUMO-tagged rat syt1 (96-421) I367C

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt1 (96-421) I367C (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    C277A; I367C

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET SUMO-syt7 (136-403)

    Plasmid

    #213621

    Purpose

    Bacterial expression of SUMO-tagged mouse syt7 (136-403)

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt7 (136-403) (Syt7 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET SUMO-syt1 5W

    Plasmid

    #213623

    Purpose

    Bacterial expression of SUMO-tagged rat syt1 5W

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt1 5W (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    M173W, F231W, F234W, C277A, V304W, Y364W, I367C

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET SUMO-syt1 s7+

    Plasmid

    #213624

    Purpose

    Bacterial expression of SUMO-tagged rat syt1 s7+

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt1 s7+ (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    L171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET SUMO-syt7 C2AB - 4A

    Plasmid

    #213625

    Purpose

    Bacterial expression of SUMO-tagged mouse syt7 C2AB - 4A

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt7 C2AB - 4A (Syt7 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    F167A, F229A, I298A, L361A

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET SUMO-syt7 C2A-2A 2B

    Plasmid

    #213626

    Purpose

    Bacterial expression of SUMO-tagged mouse syt7 C2A-2A 2B

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt7 C2A-2A 2B (Syt7 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    F167A, F229A, R363H

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET SUMO-syt7 C2AB-2A

    Plasmid

    #213627

    Purpose

    Bacterial expression of SUMO-tagged mouse syt7 C2AB-2A

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt7 C2AB-2A (Syt7 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    I298, L361A

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge