Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 17,163 results for: EMB

Showing: 9061 - 9080 of 17163 results

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pET SUMO-syt1 (96-421) I367C

Plasmid

#213620

Purpose

Bacterial expression of SUMO-tagged rat syt1 (96-421) I367C

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 (96-421) I367C (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

C277A; I367C

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt7 (136-403)

Plasmid

#213621

Purpose

Bacterial expression of SUMO-tagged mouse syt7 (136-403)

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt7 (136-403) (Syt7 Mouse)

Tags

SUMO

Expression

Bacterial

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 5W

Plasmid

#213623

Purpose

Bacterial expression of SUMO-tagged rat syt1 5W

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 5W (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

M173W, F231W, F234W, C277A, V304W, Y364W, I367C

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 s7+

Plasmid

#213624

Purpose

Bacterial expression of SUMO-tagged rat syt1 s7+

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 s7+ (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

L171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt7 C2AB - 4A

Plasmid

#213625

Purpose

Bacterial expression of SUMO-tagged mouse syt7 C2AB - 4A

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt7 C2AB - 4A (Syt7 Mouse)

Tags

SUMO

Expression

Bacterial

Mutation

F167A, F229A, I298A, L361A

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt7 C2A-2A 2B

Plasmid

#213626

Purpose

Bacterial expression of SUMO-tagged mouse syt7 C2A-2A 2B

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt7 C2A-2A 2B (Syt7 Mouse)

Tags

SUMO

Expression

Bacterial

Mutation

F167A, F229A, R363H

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt7 C2AB-2A

Plasmid

#213627

Purpose

Bacterial expression of SUMO-tagged mouse syt7 C2AB-2A

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt7 C2AB-2A (Syt7 Mouse)

Tags

SUMO

Expression

Bacterial

Mutation

I298, L361A

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-FL-syt1 5W

Plasmid

#213629

Purpose

Bacterial expression of SUMO-tagged rat FL-syt1 5W

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt1 5W (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

M173W, F231W, F234W, C277A, V304W, Y364W, I367C

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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(R)pGPD1(D)_P1(SBE109)

Plasmid

#194993

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(R) pGPD (D)

Expression

Bacterial

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TST-EGFP-GPIDAF

Plasmid

#213708

Purpose

For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an hPGK promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

hPGK

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TST-EGFP-GPIBY55

Plasmid

#213707

Purpose

For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an hPGK promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

hPGK

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pL0_EF-Fld

Plasmid

#202181

Purpose

Level 0 plasmid containing a terminator sequence from gene 23658 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Fld terminator

Use

Synthetic Biology

Mutation

None

Available Since

Feb. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR-D-Topo-kcnk17

Plasmid

#208999

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk17 (kcnk17 Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk5a

Plasmid

#208988

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk5a (kcnk5a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk6

Plasmid

#208990

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk6 (kcnk6 Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk9

Plasmid

#208992

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk9 (kcnk9 Zebrafish)

Use

Cloning

Mutation

Without STOP codon

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk13a

Plasmid

#208996

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk13a (kcnk13a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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RGG-BcLOV-mCh-EGFR

Plasmid

#208280

Purpose

Expresses RGG-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

RGG-BcLOV-mCh-EGFR

Use

Lentiviral

Mutation

H225N in mCherry- please see depositor comment

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-B'-l2-B'

Plasmid

#209119

Purpose

Bacterial expression of CC-GEMS ligand SUMO-B'-l2-B'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-B'-l2-B'

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'-l3-A'

Plasmid

#209118

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l3-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l3-A'

Expression

Bacterial

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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Showing: 9061 - 9080 of 17163 results

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