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pET28A-p53 (1-73) (P12A P13A)

Plasmid

#62066

Purpose

Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P12A, P13A

Promoter

T7

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223_OTUD6A_WT

Plasmid

#81813

Purpose

Gateway Donor vector containing OTUD6A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OTUD6A (OTUD6A Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry IL36RN S113L

Plasmid

#58321

Purpose

entry vector for human IL36RN S113L mutant

Depositor

Francesca Capon

Article

Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

Insert

IL36RN (IL36RN Human)

Use

Entry vector

Tags

myc-DDK

Mutation

S113L: c.338C>T, p.Ser113Leu

Promoter

CMV

Available Since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pBABE (hygro) hYAP 1-1delta

Plasmid

#124150

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_PRKCE_WT

Plasmid

#82227

Purpose

Gateway Donor vector containing PRKCE , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKCE (PRKCE Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM11_WT

Plasmid

#81784

Purpose

Gateway Donor vector containing RBM11 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RBM11 (RBM11 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pETM6-3A2-mCherry

Plasmid

#73425

Purpose

Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

Promoter 3A2 (orthogonal T7-lac variant)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

P3A2 (orthogonal T7-lac variant)

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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Rabbit Cav 2.2 W391A pMT2

Plasmid

#58734

Purpose

expression of rabbit CaV2.2 calcium channels with a mutation (W391) in the α1-interacting domain (AID) in the I-II loop of CaV2.2

Depositor

Annette Dolphin

Article

Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

Insert

Cav2.2 (CACNA1B Rabbit)

Expression

Mammalian

Mutation

W391A

Promoter

SV40

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry IL36RN R102W

Plasmid

#58323

Purpose

entry vector for human IL36RN R102W mutant

Depositor

Francesca Capon

Article

Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

Insert

IL36RN (IL36RN Human)

Use

Entry vector

Tags

myc-DDK

Mutation

R102W: c.304C>T, p.Arg102Trp

Promoter

CMV

Available Since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 (-) hYAP 1-2beta

Plasmid

#124144

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 _34-78/myc-His

Plasmid

#127496

Purpose

Expresses myc-tagged _34-78 NDUFS2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _34-78 (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _34-78

Promoter

CMV

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_SLC2A3_p.F88S

Plasmid

#81473

Purpose

Gateway Donor vector containing SLC2A3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SLC2A3 (SLC2A3 Human)

Use

Gateway entry vector

Mutation

F88S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SLC2A3_p.G109V

Plasmid

#81478

Purpose

Gateway Donor vector containing SLC2A3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SLC2A3 (SLC2A3 Human)

Use

Gateway entry vector

Mutation

G109V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SLC2A3_p.K49N

Plasmid

#81461

Purpose

Gateway Donor vector containing SLC2A3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SLC2A3 (SLC2A3 Human)

Use

Gateway entry vector

Mutation

K49N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RGR_WT

Plasmid

#81845

Purpose

Gateway Donor vector containing RGR , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RGR (RGR Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAB42_WT

Plasmid

#81796

Purpose

Gateway Donor vector containing RAB42 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAB42 (RAB42 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hE2F7mg_2ntmut

Plasmid

#73074

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns, 2…

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pDONR223_SGK1_p.A48G

Plasmid

#81430

Purpose

Gateway Donor vector containing SGK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SGK1 (SGK1 Human)

Use

Gateway entry vector

Mutation

A48G

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SGK1_p.F27L

Plasmid

#81391

Purpose

Gateway Donor vector containing SGK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SGK1 (SGK1 Human)

Use

Gateway entry vector

Mutation

F27L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_C13orf37_WT

Plasmid

#81872

Purpose

Gateway Donor vector containing MZT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

C13orf37 (MZT1 Human)

Use

Gateway entry vector

Mutation

S5G

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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