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pAAV-L7-6-DD-T6B-MUT-YFP

Plasmid

#235146

Purpose

Expresses the inducible mutant T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

DHFR-fused mutant T6B peptide

Use

AAV

Mutation

5 tryptophan residues of T6B-WT mutated

Promoter

L7-6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-pri-miR-Ctrl

Plasmid

#235158

Purpose

Expresses EGFP and a control primary transcript in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/primary transcript with control siRNA

Use

AAV

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-miR-206-sponge

Plasmid

#235154

Purpose

Expresses a EGFP-based sponge for miR-206 in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/miR-206 sponge with 12x binding sites

Use

AAV

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-miR-133-sponge

Plasmid

#235155

Purpose

Expresses a EGFP-based sponge for miR-133 in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/miR-133 sponge with 12x binding sites

Use

AAV

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-L7-6-FLAG-HA-T6B-MUT-YFP

Plasmid

#235142

Purpose

Expresses the mutant T6B peptide fused with YFP under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

mutant T6B peptide

Use

AAV

Tags

FLAG/HA

Mutation

5 tryptophan residues of T6B-WT mutated

Promoter

L7-6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVEA3-Tr

Plasmid

#234798

Purpose

Mammalian expression of truncated bovine arrestin-3 (1-393)

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

truncated (1-393)

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO_24xGCN4_PCC_24xPP7

Plasmid

#236094

Purpose

Constitutively encodes 24xGCN4-PCC-24xPP7 stem loops, for visualization of the Pcc mRNA.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

24xGCN4-PCC-24xPP7

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO_24xGCN4_MUT_24xPP7

Plasmid

#236093

Purpose

Constitutively encodes 24xGCN4-MUT-24xPP7 stem loops, for visualization of the Mut mRNA.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

24xGCN4-MUT-24xPP7

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_GFP

Plasmid

#235611

Purpose

Encodes GFP. For use in transfections as a negative control without epitope tags.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

GFP

Expression

Mammalian

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_GFP

Plasmid

#235632

Purpose

Encodes V5-GFP with an N-terminal nuclear export sequence. Used as a no-ALIBi negative control.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

GFP

Tags

NES-V5

Expression

Mammalian

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-CFAPorig

Plasmid

#221092

Purpose

FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pET-His-SUMO-LS4

Plasmid

#228450

Purpose

LS4 protein expression vector for bacteria

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

LS4

Tags

10xHis-SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET-His-SUMO-LS12

Plasmid

#228451

Purpose

LS12 protein expression vector for bacteria

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

LS12

Tags

10xHis-SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-CS1M2b-3c-ON8-EGFP

Plasmid

#228452

Purpose

LS4-responsive riboswitch

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

CS1M2b-3c-ON8 riboswitch-regulated EGFP

Use

Synthetic Biology

Promoter

T7

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-CS2-3c-ON8-EGFP

Plasmid

#228453

Purpose

LS12-responsive riboswitch

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

CS2-3c-ON8 riboswitch-regulated EGFP

Use

Synthetic Biology

Promoter

T7

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-NFAPorig

Plasmid

#221091

Purpose

FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-NFAPorig

Plasmid

#221086

Purpose

FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-TEF1pr-CFAPorig

Plasmid

#221087

Purpose

FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pmirGLO-mir302-8mer4x (pEZ185)

Plasmid

#229059

Purpose

Dual-luciferase reporter containing 4 copies of the 8mer site of human miR-302a

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Insert

Human miR-302a binding sites

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCI5-CasRx-NLS-HA (pKW919)

Plasmid

#229047

Purpose

Mammalian expression of CasRx-NLS-HA

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Insert

CasRx (RfxCas13d)

Use

CRISPR

Tags

NLS and NLS-HA

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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