We narrowed to 10,590 results for: Kars;

1. GGDest2-Amp

   Plasmid

   #157650

   Purpose

   Golden Gate assembly of a pET28-compatible fusion-protein ORF insert

   Depositor

   Karmella Haynes

   Article

   Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. GGDest3-Amp

   Plasmid

   #157651

   Purpose

   Golden Gate assembly of a pTXB1 compatible fusion-protein ORF insert

   Depositor

   Karmella Haynes

   Article

   Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. psubCMV-WPRE-IL3SP-mVEGF-D-dNdC

   Plasmid

   #119229

   Purpose

   Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.

   Depositor

   Kari Alitalo

   Insert

   IL3SP-Vegfd-dNdC (Vegfd Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. SLC9A3R2

   Plasmid

   #38926

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SLC9A3R2 (NHERF2 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pSB819-PKR-gor

   Plasmid

   #20028

   Depositor

   Harmit Malik

   Article

   Elde et al Nature. 2008 Nov 30. ():.

   Insert

   Protein kinase R
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

6. AAV-CAG-GFP

   Plasmid

   #28014

   Depositor

   Karel Svoboda

   Article

   Mao et al Neuron. 2011 Oct 6;72(1):111-23.

   Insert

   eGFP
   

   Use

   AAV; Adeno associate viral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-fDIO-JRGECO1a

   Plasmid

   #128317

   Purpose

   Expresses JRGECO1a under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

   Depositor

   Patricia Jensen

   Has Service

   AAV1

   Insert

   NES-JRGECO1a
   

   Use

   AAV

   Tags

   His Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1+ eiF4A1 myc HIS

   Plasmid

   #71657

   Purpose

   mammalian expression of eiF4A1

   Depositor

   Gideon Dreyfuss

   Insert

   eiF4A1 (EIF4A1 Human)
   

   Use

   Tags

   HIS and myc

   Expression

   Mammalian

   Mutation

   aa403 from Ala to Arg

   Promoter

   CMV

   Available since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FUW-hR3-EGFP

   Plasmid

   #119231

   Purpose

   Plasmid encodes full-length human VEGFR-3 gene fused in-frame with EGFP cloned in lentiviral vector.

   Depositor

   Kari Alitalo

   Insert

   VEGFR3-EGFP (FLT4 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   P hUbC

   Available since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51085

    Purpose

    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde and AAV1

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin1

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

    Plasmid

    #51084

    Purpose

    Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde and AAV1

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin1

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. PERK 7: PERK.K618A.9E10.pBABE

    Plasmid

    #21820

    Depositor

    David Ron

    Insert

    PERK K618A (Eif2ak3 Mouse)
    

    Use

    Retroviral

    Tags

    Myc (9E10)

    Expression

    Mammalian

    Mutation

    kinase dead K618A mutant

    Promoter

    Available since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTXB1-sfGFP

    Plasmid

    #51562

    Purpose

    Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)

    Depositor

    Francisco Malagon, Sankar Adhya

    Insert

    superfolder GFP
    

    Use

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBI/Rabaptin-5

    Plasmid

    #171851

    Purpose

    inducible expression of Rabaptin-5 in mammalian cells via co-transfection with pTet-Off.

    Depositor

    Guangpu Li

    Article

    Zhu et al PLoS One. 2010 Feb 16;5(2):e9226. doi: 1

    Insert

    Rabaptin-5 (human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. hSyn-FAS-DO-ReaChR-mCitrine-WPRE

    Plasmid

    #64035

    Purpose

    Expresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructs

    Depositor

    Karel Svoboda

    Insert

    hSyn-FAS-DO-ReaChR-mCitrine-WPRE
    

    Use

    AAV

    Tags

    ReaChR tagged with mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pLentiN

    Plasmid

    #37444

    Purpose

    Control lentiviral plasmid containing FLAG-HA tags

    Depositor

    Karl Munger

    Article

    Spangle et al J Virol. 2012 May 2.

    Insert

    FLAG-HA
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pB-actin E6 E7

    Plasmid

    #13712

    Depositor

    Karl Munger

    Article

    Münger et al J Virol. 1989 Oct . 63(10):4417-21.

    Insert

    HPV 16 E6 E7 (E6, E7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-GB2

    Plasmid

    #125710

    Purpose

    Eukaryotic expression of the GB2 subunit of the metabotropic GABA receptor

    Depositor

    Kai Johnsson

    Article

    Masharina et al J Am Chem Soc. 2012 Nov 21;134(46):19026

    Insert

    GB2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-IV-3xFLAG/HA/NBL10

    Plasmid

    #110568

    Purpose

    Cre-dependently expresses large ribosomal subunit protein RPL10A with a 3xFLAG/HA tag

    Depositor

    Alexander Nectow, Jeffrey Friedman

    Insert

    3xFLAG/HA-tagged anti-GFP VHH
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV Ef1a-mRuby3-WPRE

    Plasmid

    #135427

    Purpose

    Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

    Depositor

    Rylan Larsen

    Insert

    mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge