Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 14,955 results for: flag

Clear keywords
Showing: 9161 - 9180 of 14955 results

  1. pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid
    #182685
    Purpose
    Encodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    mCherry
    Expression
    Mammalian
    Promoter
    CMV and NA
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182685-plasmid-map-sequence-id-363019

    Enlarge

  2. psiCheck2-SV40p-5'UTR-mtIF3(SR)-3xFLAG-3'UTR-CMVp-mito-mTFP1

    Plasmid
    #182378
    Purpose
    Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters
    Depositor
    Chunghun Lim
    Publication
    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1
    Inserts
    mtIF3 (Mtif3 Mouse)
    mito-mTFP1
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Changed CDS sequence from 'ccacgttcaagtcacga…
    Promoter
    CMV promoter and SV40 promoter
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    182378-plasmid-map-sequence-id-358087

    Enlarge

  3. AiP13657 - pAAV-AiE0655m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3657)

    Plasmid
    #230163
    Purpose
    AiE0655m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Allen Institute for Brain Science, Boaz Levi
    Publication
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Promoter
    minBG
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    230163-plasmid-map-sequence-id-461888

    Enlarge

  4. AiP13635 - pAAV-AiE0387m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3635)

    Plasmid
    #230159
    Purpose
    AiE0387m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Allen Institute for Brain Science, Boaz Levi
    Publication
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Promoter
    minBG
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    230159-plasmid-map-sequence-id-461884

    Enlarge

  5. AiP13294 - pAAV-AiE0940m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3294)

    Plasmid
    #230144
    Purpose
    AiE0940m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Allen Institute for Brain Science, Boaz Levi
    Publication
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Promoter
    minBG
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    230144-plasmid-map-sequence-id-461871

    Enlarge

  6. AiP13295 - pAAV-AiE0941m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3295)

    Plasmid
    #230145
    Purpose
    AiE0941m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Allen Institute for Brain Science, Boaz Levi
    Publication
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Promoter
    minBG
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    230145-plasmid-map-sequence-id-461872

    Enlarge

  8. AiP13655 - pAAV-AiE0552h-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3655)

    Plasmid
    #224035
    Purpose
    AiE0552h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_IT and L6_IT neurons
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Publication
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Mutation
    NA
    Promoter
    minBG
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    224035-plasmid-map-sequence-id-449734

    Enlarge

  11. AiP13445 - pAAV-AiE0873m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3445)

    Plasmid
    #191725
    Purpose
    AI plasmid ALIAS: AiP13445. Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic neurons
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Publication
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Mutation
    none
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    191725-plasmid-map-sequence-id-376590

    Enlarge

  12. pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE

    Plasmid
    #198054
    Purpose
    All-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloning
    Depositor
    Lacra Bintu
    Publication
    Ludwig et al Cell Syst. 2023 Jun 21;14(6):482-500.e8.
    Inserts
    rTetR(SE-G72P)-VP48-T2A-mCherry-BSD
    3xFLAG-insert
    Use
    Lentiviral and Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    TRE3G and pEF
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    198054-plasmid-map-sequence-id-391373

    Enlarge

  13. pcDNA5-FRT-TO-FH-Nprot

    Plasmid
    #157729
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Publication
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FH-Nprot (N SARS-CoV-2)
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized
    Available since
    Aug. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157729-plasmid-map-sequence-id-307938

    Enlarge

  15. pcDNA5-FRT-TO-FH-Nsp10

    Plasmid
    #157707
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Publication
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FH-Nsp10 (ORF1ab SARS-CoV-2)
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157707-plasmid-map-sequence-id-307872

    Enlarge

  16. pcDNA5-FRT-TO-FH-Nsp9

    Plasmid
    #157704
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Publication
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FH-Nsp9 (ORF1ab SARS-CoV-2)
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157704-plasmid-map-sequence-id-307868

    Enlarge

  19. VRQR-HF1 variant

    Plasmid
    #138563
    Purpose
    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRQR-HF1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138563-plasmid-map-sequence-id-274789

    Enlarge

  20. QQR1 variant

    Plasmid
    #138564
    Purpose
    Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    QQR1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138564-plasmid-map-sequence-id-274793

    Enlarge

Showing: 9161 - 9180 of 14955 results