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pCfB3045(gRNA XI-3)
Plasmid#73287PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKG I alpha dNPS
Plasmid#16391DepositorInsertPKG1 alpha (PRKG1 Human)
UseTagsHAExpressionMammalianMutationN-terminal pseudosubstrate domain deletion: delet…PromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEPQD2KN0908
Plasmid#177088PurposeMoClo Level 2 plasmid containing multiple transcriptional units for transient expression of P19 (cytosol, driven by nosP), along with CrDXS2, PaGPPS1, and CrGES (plastid, driven by 4x opaatB)DepositorInsertP19, CrDXS2, PaGPPS1, and CrGES
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS1398
Plasmid#205971PurposeMWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1DepositorInsertCas5-HNH crRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1182
Plasmid#205964PurposeNZ_CP025815 DinG HNH crRNA expression in pCOLADuet-1DepositorInsertDinG HNH crRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-sh-mFAK C
Plasmid#37015DepositorInsertsh-mFAK C (Ptk2 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceMay 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pU6-Decoy
Plasmid#80324PurposeEncodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondiiDepositorInsertDecoy sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
GOLPH3 shRNA
Plasmid#163860PurposeshRNA targeting the coding sequences of GOLPH3DepositorInsertgolgi phosphoprotein 3 (GOLPH3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT005
Plasmid#182715PurposeConstituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator regionDepositorInsertCasRx crRNA cloning backbone
UseCRISPRTagsExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)Available sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSMP-G9A_1
Plasmid#36395DepositorInsertG9A (EHMT2 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO-STAG2 shRNA 3782
Plasmid#31979DepositorInsertSTAG2 (STAG2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_LMTK2
Plasmid#187752PurposeMAC-tagged gene expression of human LMTK2DepositorInsertLMTK2 (LMTK2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-V2_hMSH2
Plasmid#186155PurposesgRNA targeting human MSH2 geneDepositorInsertMSH2 (MSH2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD2KN0897
Plasmid#177090PurposeMoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H driven by 4x opaatBDepositorInsertCrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_TFRC_B
Plasmid#72626PurposeExpresses two gRNAs targeting the TFRC promoterDepositorInsertgRNAs toward TFRC
UseTagsExpressionMammalianMutationPromoterU6 (gRNA1) and H1 (gRNA2)Available sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSMP-Dot1L_1
Plasmid#36339DepositorInsertDot1L (DOT1L Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1.2
Plasmid#32468DepositorInsertG-GECO1.2
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…PromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2 sgSp1
Plasmid#174907PurposeSecond generation sgRNA against Sp1DepositorInsertSpecificity Protein 1 (SP1 Human)
UseTagsExpressionMammalianMutationMissense mutation to mutate PAM sequencePromoterEFS-NSAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shTGFBR3 puro
Plasmid#58696PurposeLentiviral shRNA vector for knockdown of human TGFBR3DepositorInsertshTGFBR3 (TGFBR3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
LEP-shMLL-AF9.1039
Plasmid#105566Purposeretrovirally express MLL-AF9 shRNA with puro resistance and GFP markerDepositorInsertshRNA targeting MLL part of MLL-AF9 fusion
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
LEP-shMLL-AF9.643
Plasmid#105565Purposeretrovirally express MLL-AF9 shRNA with puro resistance and GFP markerDepositorInsertshRNA targeting MLL part of MLL-AF9 fusion
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)
Plasmid#164040PurposeExpression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2DepositorInsertsgTS5-sgTS6-sgTS7
UseCRISPRTagsExpressionMutationPromotermouse U6Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
SL2-sgTelo-MTSa/BFP/pdCas9-C1
Plasmid#162760PurposeExpressing dCas9 and sgRNA containg MTSa targeting telomeresDepositorInsertdCas9 and sgRNA(SL2-sgTelo-MTSa)
UseTagsExpressionMammalianMutationdCas9(nuclease deactivated Cas9)PromoterU6/CMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TO-HA-SH3BP1 (RNAi-res)
Plasmid#136645PurposeExpression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUCDepositorInsertSH3BP1 (RNAi resistant) (SH3BP1 Human)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationGATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTCPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-non-target
Plasmid#129465PurposeDerived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.DepositorInsertNon-target/random gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
CEBPalpha NGFR
Plasmid#44627DepositorInsertCEBPalpha (Cebpa Mouse)
UseRetroviralTagsIRES and truncated NGFRExpressionMammalianMutationPromoterAvailable sinceApril 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBLO2 casX
Plasmid#123122PurposeE. coli expression vector for CasX sgRNADepositorInsertCasX guide RNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2SCb
Plasmid#59385PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.DepositorInsertTT-ISceI-cat2
UseTemplateTagsExpressionMutationThe 3’-end of the chloramphenicol resistance gene…PromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)
Plasmid#136646PurposeExpression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUCDepositorInsertSH3BP1R312A (RNAi resistant) (SH3BP1 Human)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationGATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…PromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
SL2-sgTelo-MTSa/EGFP/pdCas9-C1
Plasmid#162759PurposeExpressing dCas9 and sgRNA containg MTSa targeting telomeresDepositorInsertdCas9 and sgRNA(SL2-sgTelo-MTSa)
UseTagsExpressionMammalianMutationdCas9(nuclease deactivated Cas9)PromoterU6/CMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
BoxB-MS2 adRNA (RAB7A-ACG site)
Plasmid#170155PurposeAAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editingDepositorInsertBoxB-RAB7A(ACG)-MS2
UseAAVTagsExpressionMammalianMutationPromoterHuman U6Available sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1.mir451_KO
Plasmid#164689PurposeFor CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNADepositorInsertmiR-451 CRISPR KO gRNA (MIR451A )
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 shRNA against CRTC2
Plasmid#72711PurposeLentiviral expression of shRNA against CRTC2 (3rd generation)DepositorInsertCRTC2
UseLentiviralTagsCMV-EGFPExpressionMammalianMutationPromoterU6Available sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-B-GECO1
Plasmid#32469DepositorInsertB-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
GFP-GLT1a-V5
Plasmid#162517PurposeRat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loopDepositorInsertGLT1a (Slc1a2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-GLT1b-V5
Plasmid#162518PurposeRat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loopDepositorInsertGLT1b (Slc1a2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pSUPERIOR.puro-shp53
Plasmid#38035DepositorInsertshp53 (TP53 Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gLacZ.hSyn.flex.H2B.RFP
Plasmid#170373PurposeNegative control guide, Cre-inducible plasmid expressing nuclear RFP.DepositorInsertCre inducible H2B RFP
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only
