Addgene

We narrowed to 37,652 results for: KAN

Showing: 2251 - 2300 of 37652 results

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pGL1_46

Plasmid

#199053

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

Dummy ORI plus NGS barcode 23

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGL1_40

Plasmid

#199047

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pWKS1 ORI plus NGS barcode 17

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGL1_2

Plasmid

#199034

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pUB110 ORI plus NGS barcode 2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGL1_45

Plasmid

#199052

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pNG2 ORI plus NGS barcode 22

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGLDO_1 [α-sfYFP-β]

Plasmid

#199032

Purpose

Level 1 plasmid, methylated YFP dropout

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

α-sfYFP-β dropout part

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGL1_44

Plasmid

#199051

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1-UP ORI plus NGS barcode 21

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGL1_9

Plasmid

#199041

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSC101ts ORI plus NGS barcode 10

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL1_1

Plasmid

#199033

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAL5000 ORI plus NGS barcode 1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL1_47

Plasmid

#199054

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

p15A ORI plus NGS barcode 24

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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TM SE T206A

Plasmid

#79664

Purpose

More dimeric SE attached to Triple Mutant GEVI from Piao et al 2015

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

Triple Mutant SE T206A

Use

Tags

Expression

Mammalian

Mutation

T206A

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pAXY0001

Plasmid

#179834

Purpose

binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

Depositor

Xiaohan Yang

Article

Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

Insert

eYGFPuv

Use

Binary vector for plant transformation

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pEEV2/11

Plasmid

#166888

Purpose

Helper/packaging plasmid for production of AAV2/11 pseudotype vector

Depositor

Seiichiro Mori, Tadahito Kanda

Article

Mori et al Virology. 2004 Dec 20;330(2):375-83. doi

Insert

AAV2 rep/AAV11 cap

Use

AAV

Tags

Expression

Mutation

Promoter

AAV2 p5

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pGFPmAID

Plasmid

#174213

Purpose

GFP::mAID donor backbone

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pTN14

Plasmid

#178945

Purpose

Expresses AtTIR1(F79G) in C elegans

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Insert

AtTIR1(F74G)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMK344 (Hygro-P2A-mAID)

Plasmid

#121178

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
LM627

Plasmid

#191551

Purpose

LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

Depositor

David Baker

Article

Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

Insert

ccdb

Use

Tags

SNAC-tag, HIS-tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FAST-p10

Plasmid

#89152

Purpose

Express p10-FAST in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

NBV p10-FAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promotor

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLII12Pglu-700uDelta6

Plasmid

#17866

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLII12Pglu-700uDelta6

Use

Tags

Citrine

Expression

Mammalian

Mutation

700uDelta6, ECFP insert in mglB

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pMK232 (CMV-OsTIR1-PURO)

Plasmid

#72834

Purpose

Expresses OsTIR1 under the control of the CMV promoter

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

AAVS1-CMV-OsTIR1-PURO-AAVS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMK231 (AAVS1 CMV-MCS-PURO)

Plasmid

#105924

Purpose

Donor for integration at the human AAVS1 locus

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TR-Csy4

Plasmid

#80601

Purpose

Expresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CBA

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2

Plasmid

#18047

Depositor

Edward Yeh

Article

Kang et al Mol Cell. 2010 Apr 23. 38(2):191-201.

Insert

SENP2 (SENP2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
LM670

Plasmid

#191552

Purpose

LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

Depositor

David Baker

Article

Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

Insert

ccdb

Use

Tags

HIS-tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC-DEST-hPROX1-tdTomato-PuroTK

Plasmid

#141027

Purpose

Reporter integration

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMK347 (BSD-P2A-mAID)

Plasmid

#121181

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

BSD-P2A-mAID

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK391 (mAID-BSD)

Plasmid

#121192

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-BSD

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-D1R

Plasmid

#89160

Purpose

Express vaccinia virus D1R in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Vaccinia virus D1R

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promotor

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
P16ΔS:S10-(MCS)

Plasmid

#108182

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

P16ΔS

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAXY0002

Plasmid

#179835

Purpose

binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

Depositor

Xiaohan Yang

Article

Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

Type

Empty backbone

Use

Binary vector for plant transformation

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB1620 hU6-BpiI-Cas13bt3-DR

Plasmid

#176315

Purpose

hU6-BpiI-Cas13bt3-DR (crRNA expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

Cas13bt3 crRNA direct repeat

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQAT03

Plasmid

#223375

Purpose

T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-SpCas9-AtU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Mutation

Promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
gTEMP_pcDNA3

Plasmid

#89583

Purpose

Genetically encoded ratiometric fluorescent thermometer

Depositor

Takeharu Nagai

Article

Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

Insert

gTEMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-Neq2X7

Plasmid

#182366

Purpose

Expresses Neq2X7 DNA polymerase

Depositor

Morten Norholm

Article

Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

Insert

Neq2X7 DNA polymerase

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK393 (mAID-mCherry-BSD)

Plasmid

#121194

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-mCherry-BSD

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-VP3SA11

Plasmid

#89164

Purpose

Express Rotavirus SA11 VP3 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP3

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-NSP4SA11

Plasmid

#89171

Purpose

Express Rotavirus SA11 NSP4 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP4

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-VP4SA11

Plasmid

#89165

Purpose

Express Rotavirus SA11 VP4 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP4

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbB19k-GFP

Plasmid

#126135

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23105

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
hROSA26 CRISPR-pX330

Plasmid

#105927

Purpose

hROSA26 targeting CRISPR plasmid

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

hROSA26 gRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-NSP3SA11

Plasmid

#89170

Purpose

Express Rotavirus SA11 NSP3 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP3

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-NSP1SA11

Plasmid

#89168

Purpose

Express Rotavirus SA11 NSP1 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-NSP5SA11

Plasmid

#89172

Purpose

Express Rotavirus SA11 NSP5 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP5

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-NSP2SA11

Plasmid

#89169

Purpose

Express Rotavirus SA11 NSP2 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-VP6SA11

Plasmid

#89166

Purpose

Express Rotavirus SA11 VP6 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP6

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-VP7SA11

Plasmid

#89167

Purpose

Express Rotavirus SA11 VP7 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP7

Use

Tags

*see below

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pYTK-DN4

Plasmid

#180285

Purpose

KanR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - KanR - CEN6/ARS4

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mito-gTEMP_pcDNA3

Plasmid

#109117

Purpose

Mitochondria targetting gTEMP

Depositor

Takeharu Nagai

Article

Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

Insert

mito-gTEMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GLUT1-eGFP/pcDNA-DEST47

Plasmid

#18729

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

pcDNA-DEST47 /hGLUT1-eGFP

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted TAA

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pBM-strep-UCP1

Plasmid

#216207

Purpose

for expression of human strep-tagged UCP1 protein in BacMam system

Depositor

Lei Chen

Article

Kang et al Nature. 2023 Aug;620(7972):226-231. doi:

Insert

UCP1 (UCP1 Human)

Use

Tags

strep

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTJV1ScKm-rpoB

Plasmid

#166980

Purpose

Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Insert

NeoR/KanR

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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