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NM-01
Plasmid#69799PurposeBacterial expression of Cas9 with His, MBP, and Strep tagsDepositorInsertCas9
UseTagsHIS, MBP, and StrepIIExpressionBacterialMutationAspartate 10 to Alanine (D10A) and Histidine 840 …PromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJK633
Plasmid#72397PurposeProduces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)DepositorInsertphosphoribosylaminoimidazole synthetase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pNB3033
Plasmid#113397PurposeTy3 Gag3-His tagged, codon optimized for expression in E. coli; . IPTG inducibleDepositorInsertTy3 Gag3 codon optimized for E. coli expression
UseTagsGag3-6XHis tagExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pJK647
Plasmid#71701PurposeProduces E. coli thioredoxin reductase (EcTrxB)DepositorInsertthioredoxin reductase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pJK408
Plasmid#72422PurposeProduces Acetobacter aceti 1023 Est10, C-terminal His5 tagDepositorInsertferuloyl esterase
UseTagsHis5ExpressionBacterialMutationPromoterT7Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pJK367
Plasmid#72406PurposeProduces Acetobacter aceti 1023 Est06DepositorInsertlipase
UseTagsExpressionBacterialMutationlacks Met1 and Thr2 in annotated (v1) sequencePromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK402
Plasmid#72415PurposeProduces Acetobacter aceti 1023 Est03, N-terminal His6 tagDepositorInsert4-hydroxybenzoyl-CoA thioesterase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK580
Plasmid#71694PurposeProduces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 tag (AaTrxAH6)DepositorInsertthioredoxin
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
UBC45
Plasmid#25469PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertubc45.001.200
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
4ROJ
Plasmid#64661PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertVAV2:JMC092-C08:C231327 (VAV2 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceMay 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pJK532
Plasmid#72513PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit with N-terminal His6 tag (H6AaPurQ)DepositorInsertformylglycinamidine ribonucleotide synthetase, glutaminase subunit
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMarch 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
LOC201475
Plasmid#25512PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertLOC201475
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
Top7 K39E K40E V48V K55E
Plasmid#12464PurposePlasmid contains Top7 K39E K40E V46A and K55E. Please note this is DIFFERENT than what the plasmid name implies.DepositorInsertTop7 K39E K40E V46A K55E
UseTags6xHisExpressionBacterialMutationK39E K40E V46A K55EPromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
UBC44
Plasmid#25468PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertubc44.001.167
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pAIP4_1AKZ
Plasmid#234172PurposeHeterologous protein expression of uracil-DNA glycosylase (UDG) in Escherichia coliDepositorInsert1AKZ (UNG Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 10, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_2ESK
Plasmid#234232PurposeHeterologous protein expression of ubiquitin-conjugating enzyme E2 D2 in Escherichia coliDepositorInsert2ESK (UBE2D2 Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_4J5Q
Plasmid#234233PurposeHeterologous protein expression of ADP-ribose glycohydrolase OARD1 in Escherichia coliDepositorInsert4J5Q (tas3 Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_3OBS
Plasmid#234227PurposeHeterologous protein expression of tumor susceptibility gene 101 protein in Escherichia coliDepositorInsert3OBS (TSG101 Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_2WWE
Plasmid#234210PurposeHeterologous protein expression of PI3K-C2-gamma in Escherichia coliDepositorInsert2WWE (PIK3C2G Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_1JL1
Plasmid#234206PurposeHeterologous protein expression of ribonuclease HI (RNase HI) in Escherichia coliDepositorInsert1JL1 (rnhA Escherichia coli)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_5GUQ
Plasmid#234171PurposeHeterologous protein expression of ASCH domain-containing ribonuclease in Escherichia coliDepositorInsert5GUQ
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_1AKO
Plasmid#234162PurposeHeterologous protein expression of exodeoxyribonuclease III (Exonuclease III) in Escherichia coliDepositorInsert1AKO (xthA Escherichia coli K-12)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_3V46
Plasmid#234165PurposeHeterologous protein expression of cell division control protein 73 in Escherichia coliDepositorInsert3V46 (CDC73 Budding Yeast)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
TBXTA-c005
Plasmid#139754PurposeFor protein expression/crystallography (PDB:6F58)DepositorInsertTBXT, DNA-binding domain, WT (TBXT Human)
UseTagsHis6ExpressionBacterialMutationContains only amino acids E41-S224PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK561
Plasmid#72283PurposeProduces Acetobacter aceti 1023 thioredoxin with Cys35>Ser mutant (AaTrxA-C35S)DepositorInsertthioredoxin
UseTagsExpressionBacterialMutationchanges cysteine-35 to serinePromoterT7Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK523
Plasmid#72442PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes, with N-terminal His6 tag (H6AaOrfX)DepositorInserthypothetical protein
UseTagsHis6ExpressionBacterialMutationsynthetic gene with ~40 silent substitutionsPromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK126
Plasmid#73337PurposeEncodes Mycobacterium tuberculosis FdxB (C-terminal flavoprotein reductase domain)DepositorInsertelectron transfer flavoprotein
UseTagsHis6ExpressionBacterialMutationencodes residues 328-685PromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK366
Plasmid#72405PurposeProduces Acetobacter aceti 1023 Est02DepositorInsertpeptidase S9
UseTagsExpressionBacterialMutationadditional N-terminal MVKNGS sequence, alternate …PromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK377
Plasmid#72411PurposeProduces Acetobacter aceti 1023 Est05DepositorInserthypothetical protein
UseTagsExpressionBacterialMutationadditional N-terminal MTFLYSICHIVMNIVSSTPHFNVWICF…PromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK614
Plasmid#72387PurposeProduces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)DepositorInsertphosphoribosylamine-glycine ligase
UseTagsHis6ExpressionBacterialMutationcontains 4 silent mutations in codons 9-11 and 42…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK621
Plasmid#72388PurposeProduces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)DepositorInsertphosphoribosylamine-glycine ligase
UseTagsHis6ExpressionBacterialMutationcontains 3 silent mutations in codons 9-11 to fac…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK592
Plasmid#72386PurposeProduces Escherichia coli glycinamide ribonucleotide synthetase (EcPurD)DepositorInsertphosphoribosylamine-glycine ligase
UseTagsExpressionBacterialMutationcontains 3 silent mutations in codons 9-11 to fac…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK684
Plasmid#71638PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Val mutant (TdPurE2-S38V)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges serine-38 to valinePromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK685
Plasmid#71639PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asn mutant (TdPurE2-S38N)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges serine-38 to asparaginePromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK687
Plasmid#71640PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met mutant (TdPurE2-K41M)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges lysine-41 to methioninePromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK688
Plasmid#71641PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Arg mutant (TdPurE2-K41R)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges lysine-41 to argininePromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only
