We narrowed to 8,804 results for: App

1. pJK293

   Plasmid

   #73372

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Phe/His89>Phe mutant (AaPurE1-H59F,H89F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changed histidine-59 to phenylalanine and histidi…

   Promoter

   T7

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK294

   Plasmid

   #73373

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asp/His89>Asp mutant (AaPurE1-H59D,H89D)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changed histidine-59 to aspartate and histidine-8…

   Promoter

   T7

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK528

   Plasmid

   #71625

   Purpose

   Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met105>Ser/Ile109>Trp mutant (TdPurE2-M105S,I109W)

   Depositor

   T. J. Kappock

   Insert

   phosphoribosylaminoimidazole carboxylase
   

   Expression

   Bacterial

   Mutation

   changes methionine-105 to serine, isoleucine-109 …

   Promoter

   T7

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK439

   Plasmid

   #72478

   Purpose

   Produces Thermoplasma acidophilum citrate synthase pseudo-dimer

   Depositor

   T. J. Kappock

   Insert

   citrate synthase (artificial dimer)
   

   Expression

   Bacterial

   Mutation

   inserted NSTCLVPRGSPV sequence between C- and N-t…

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK404

   Plasmid

   #72417

   Purpose

   Produces Acetobacter aceti 1023 Est12, N-terminal His6 tag

   Depositor

   T. J. Kappock

   Insert

   acyl-CoA thioesterase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   additional N-terminal 15 residues MSETYGTGQKEQHC,…

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK268

   Plasmid

   #72436

   Purpose

   Produces Acetobacter aceti 1023 del7-GloA

   Depositor

   T. J. Kappock

   Insert

   lactoylglutathione lyase
   

   Expression

   Bacterial

   Mutation

   Missing residues 1-7

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK627

   Plasmid

   #72462

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp/Tyr154>Phe mutant (AaPurE1-S57D,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   Mutation changes serine-57 to aspartate, tyrosine…

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK628

   Plasmid

   #72463

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Ala/Tyr154>Phe mutant (AaPurE1-S57A,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   Mutation changes serine-57 to alanine, tyrosine-1…

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK573

   Plasmid

   #72456

   Purpose

   Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   synthetic gene with ~57 silent substitutions

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK632

    Plasmid

    #72465

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Val/Tyr154>Phe mutant (AaPurE1-S57V,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    Mutation changes serine-57 to valine, tyrosine-15…

    Promoter

    T7

    Available Since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK629

    Plasmid

    #72464

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Thr/Tyr154>Phe mutant (AaPurE1-S57T,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    Mutation changes serine-57 to threonine, tyrosine…

    Promoter

    T7

    Available Since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK541

    Plasmid

    #72447

    Purpose

    Produces Acetobacter aceti 1023 small hypothetical protein (AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Expression

    Bacterial

    Promoter

    T7 and n/a

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK569

    Plasmid

    #72455

    Purpose

    Produces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic gene

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    synthetic gene with ~51 silent substitutions

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK587

    Plasmid

    #71696

    Purpose

    Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag and Cys138>Ser mutant (AaTrxB1H6-C138S)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin reductase 1
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes cysteine-138 to serine

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK525

    Plasmid

    #71601

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp97>Tyr mutant (AaPurE1fyw)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, tryptopha…

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK526

    Plasmid

    #71602

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes tryptophan-97 to tyrosine, tryptophan-165…

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK601

    Plasmid

    #71606

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Tyr154>Phe mutant (AaPurE1-Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes tyrosine-154 to phenylalanine, additional…

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK698

    Plasmid

    #71616

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp165>Phe mutant (AaPurE1fwf)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, tryptopha…

    Promoter

    T5

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK507

    Plasmid

    #71597

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp165>Phe mutant (AaPurE1fwf)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, tryptopha…

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK509

    Plasmid

    #71599

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn mutant (AaPurE1fww-H59N)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, histidine…

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge