pGG426_UV5
Plasmid
#165608
Purpose
Vector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)
Depositor
Insert
lacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
Use
CRISPR and Synthetic Biology
Expression
Bacterial
Mutation
D1135V, S1136R, D1332K and R1333G mutations in Sp…
Promoter
lacUV5 driving Cas9 VRKG and UV5 driving sgRNA
Available Since
April 21, 2021
Availability
Academic Institutions and Nonprofits only
