We narrowed to 920 results for: pgem

Showing: 901 - 920 of 920 results

Results per page:

Sort results by:

pPUbc (GB0145)

Plasmid

#68170

Purpose

Provides the S. Lycopersicum Ubiquitin carrier protein promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlUbc promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p2x35S (GB0222)

Plasmid

#68173

Purpose

Provides the CaMV dual 35S Promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

2x35S promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only
pT2A11 (GB0143)

Plasmid

#68189

Purpose

Provides the S. Lycopersicum 2A11 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Sl2A11 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE8 (GB0144)

Plasmid

#68190

Purpose

Provides the S. Lycopersicum E8 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlE8 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
BtA3N

Plasmid

#238981

Purpose

In vitro transcription of bovine arrestin-3 short isoform

Depositor

Vsevolod Gurevich

Publication

Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

Insert

arrestin-3 short (ARR3 Bovine)

Use

In vitro transcription

Mutation

silent, Cla I side introduced before stop codon

Promoter

SP6

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMyb12 (GB0075)

Plasmid

#68199

Purpose

Provides the CDS of theSolanum lycopersicum Myb12 transcription factor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Myb12 coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLexAOp (GB0118)

Plasmid

#68181

Purpose

Provides the 4xLexA Operator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

4xOpLexA

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
BtA3CL

Plasmid

#238992

Purpose

In vitro transcription of mutant bovine arrestin-3 short isoform

Depositor

Vsevolod Gurevich

Publication

Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

Insert

arrestin-3 short (ARR3 Bovine)

Use

In vitro transcription

Mutation

C17S, C60 V, C126S, C141L, C151V, C243V, C252V, C…

Promoter

SP6

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD_Mini35s(-60,UTR) (GB0050)

Plasmid

#68176

Purpose

Provides the CaMV Mini35S Promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

CaMV Mini35S promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD 5'UTR:YFP:Tnos (GB1483)

Plasmid

#160557

Purpose

Part 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of YFP and NOS terminator (5'UTR:YFP:TNos)

Depositor

Diego Orzaez

Publication

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

5'UTR:YFP:Tnos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAct2 (GB0210)

Plasmid

#68191

Purpose

Provides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtAct2 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p5'UTRAct2 (GB0193)

Plasmid

#68178

Purpose

Provides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

5'UTR AtAct2

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pPAt2S3 (GB0029)

Plasmid

#68162

Purpose

Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

At2S3 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
XE51 XPKC-alpha

Plasmid

#16746

Depositor

Randall Moon

Publication

Otte et al Cell. 1992 Mar 20;68(6):1021-9. doi: 10.

Insert

XPKC-Alpha (prkca Frog)

Use

Xenopus expression

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pLexA::SRDX (GB0732)

Plasmid

#68184

Purpose

Provides the LexA Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

LexA Binding Domain fused to SRDX Transcriptional Repressor

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGal4BD::BDR (GB0734)

Plasmid

#68185

Purpose

Provides the Gal4 Binding Domain fused to the BDR Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Gal4 Binding Domain fused to BDR Transcriptional Repressor

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pG-HIV-SIN-2LTR

Plasmid

#104591

Purpose

Standard for qPCR

Depositor

Kristine Yoder

Publication

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

2LTR circle junction of self-inactivating HIV-based vectors

Use

Cloning vector

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
tRNA-gRNA position [M1_2] (GB1209)

Plasmid

#75410

Purpose

tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_2]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (3-part multiplexing)

Depositor

Diego Orzaez

Publication

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA position [M1_2]

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pVD1 tRNA-gRNA E4-En-1 (GB2243)

Plasmid

#160565

Purpose

tRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

Depositor

Diego Orzaez

Publication

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

tRNA-gRNA position E4-En-1 (Multiplexing Edit)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-loxp-stop-loxp-IL17RA-QY-tTA-T2A-IL17RC-TEV-IRES-TagBFP-PA

Plasmid

#255643

Purpose

Express mouse IL-17A CyCLoPs cassette followed by TagBFP as a marker

Depositor

Jun Huh

Publication

Lu et al Cell. 2026 Feb 5;189(3):939-955.e26. doi

Inserts

IL-17RA (Il17ra Mouse)
IL-17RC (Il17rc Mouse)
TagBFP

Tags

TEV and tTA

Expression

Mammalian

Promoter

CAG

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge