We narrowed to 63,772 results for: cell

1. pUCM-CLYBL-NGN2-BRN3A

   Plasmid

   #165597

   Purpose

   Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.

   Depositor

   Carsten Bönnemann

   Article

   Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   BRN3A (POU4F1 Human)
   EGFP
   Puromycin N-acetyl transferase
   rtTA3G
   SBP Delta-LNGFR
   

   Use

   CRISPR and TALEN

   Expression

   Mammalian

   Promoter

   CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…

   Available Since

   March 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pmiRA1-MYB

   Plasmid

   #141120

   Purpose

   Lentiviral overexpression of MYB in mammalian cells.

   Depositor

   Jianjun Chen

   Article

   Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

   Insert

   MYB (MYB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A)

   Plasmid

   #84777

   Purpose

   Second generation lenti expressing Mash1 and Neurogenin for iN transdifferentation to neurons

   Depositor

   Fred Gage

   Article

   Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

   Insert

   Ngn2 and Mash1 with a T2A (NEUROG2, ASCL1 Human)
   

   Use

   Lentiviral

   Promoter

   pTIGHT

   Available Since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE2-TetOminiCMV-OSKM

   Plasmid

   #136541

   Purpose

   Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4, cMyc

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Sox2, Pou5f1, Myc, Klf4 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pmiR-report-MYB-WT

   Plasmid

   #141114

   Purpose

   Reporter plasmid of WT MYB in mammalian cells.

   Depositor

   Jianjun Chen

   Article

   Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

   Insert

   CMYB (MYB Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pmiR-report-MYB-mut

   Plasmid

   #141115

   Purpose

   Reporter plasmid of mutant MYB in mammalian cells.

   Depositor

   Jianjun Chen

   Article

   Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

   Insert

   MYB (MYB Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   A to T mutations at m6A sites

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5-IMPROVED

   Plasmid

   #154088

   Purpose

   Improved version to express LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells.

   Depositor

   George Daley

   Article

   Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

   Insert

   Ligand Dependent Nuclear Receptor Corepressor, HOXA9, HOXA5 (LCOR, HOXA9, HOXA5 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Tet on

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PCDH-METTL14-R298P

   Plasmid

   #141113

   Purpose

   Overexpression of METTL14 mutant in mammalian cells.

   Depositor

   Jianjun Chen

   Article

   Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

   Insert

   HMETTL14 (METTL14 Human)
   

   Expression

   Mammalian

   Mutation

   Substitution - Missense, position 298, R➞P

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pINDUCER-21-RUNX1-P2A-ERG-IMPROVED

   Plasmid

   #154089

   Purpose

   Improved version to express RUNX1, P2A, ERG in mammalian cells.

   Depositor

   George Daley

   Article

   Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

   Insert

   Runt Related Transcription Factor 1, ETS-Related Gene (RUNX1, ERG Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Tet on

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4

    Plasmid

    #193348

    Purpose

    tetO-S*K (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Sox2-17-t2a-Klf4 (Klf4, Sox2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    TetO mini CMV

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. T7-VEE-CFP-e2a-SOX2AV-p2a-KLF4

    Plasmid

    #193359

    Purpose

    self-replicating RNA vector expressing CFP + human SOX2A61V + KLF4 + PuroR

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    CFP-e2a-SOX2AV-p2a-KLF4 (KLF4, SOX2 Human, Aequorea victoria)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Mutation

    SOX2A61V is a highly cooperative point mutant of …

    Promoter

    T7

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MSCV-PIG-METTL14-WT

    Plasmid

    #141118

    Purpose

    Retroviral overexpression of WT METTL14 in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    METTL14 (METTL14 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MSCV-PIG-METTL14-R298P

    Plasmid

    #141119

    Purpose

    Retroviral overexpression of mutant METTL14 in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    METTL14 (METTL14 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Substitution - Missense, position 298, R➞P

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. T7-VEE-CFP-e2a-SOX2-p2a-KLF4

    Plasmid

    #193358

    Purpose

    VEE-CFP-SK (self-replicating RNA vector expressing CFP + human SOX2 + KLF4 + PuroR)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    CFP-e2a-SOX2-p2a-KLF4 (KLF4, SOX2 Human, Aequorea victoria)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE2-TetOminiCMV-Klf4-IRES-Sox2-17

    Plasmid

    #193299

    Purpose

    tetO-KS* (Dox-inducible reprogramming lentiviral vector expressing mouse Klf4 + super-Sox)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Klf4-IRES-Sox2-17 (Sox2, Klf4 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    TetO mini CMV

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. px330 Gatad2a sgRNA (for deletion)

    Plasmid

    #110814

    Purpose

    Expressed sgRNA for generating Gatad2a deletion (knockout ) in mouse cell lines

    Depositor

    Jacob Hanna

    Article

    Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

    Insert

    mGataD2a (Gatad2a Mouse)
    

    Expression

    Mammalian

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. px330 Gatad2a sgRNA (for flox targeting)

    Plasmid

    #110815

    Purpose

    Used with pNTK Gatad2a flox allele targeting construct in mouse cells

    Depositor

    Jacob Hanna

    Article

    Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

    Insert

    mGataD2a (Gatad2a Mouse)
    

    Expression

    Mammalian

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ORFtag_TetR-SD_Frame2

    Plasmid

    #220982

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame2

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ORFtag_TetR-SD_Frame3

    Plasmid

    #220983

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame3

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. IDG_SCN3B_OE_1

    Plasmid

    #161625

    Purpose

    Overexpresses SCN3B

    Depositor

    Mike McManus

    Insert

    SCN3B (SCN3B Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge