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pdU6-2_sgRNA1-for-dSERT

Plasmid

#221830

Purpose

Plasmid to express gRNA1 (cgaaatctgcgctctacttg) for editing at the beginning of Drosophila SERT coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

dSERT gRNA1 (SerT Fly)

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6 promoter

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA2-for-dSERT

Plasmid

#221831

Purpose

Plasmid to express gRNA2 (gggattcgagcggcccgtcg) for editing at the beginning of Drosophila SERT coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

dSERT gRNA2 (SerT Fly)

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6 promoter

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pACUH 5-HT7R-7xGFP11-HA

Plasmid

#221836

Purpose

Plasmid for generating 10xUAS-5-HT7R-7xGFP11-HA transgenic flies with attP/attB integration. It carries the attB sequence.

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

5-HT7R (5-HT7 Fly)

Tags

7xGFP11-HA

Expression

Insect

Promoter

hsp70

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-Ace-mNeon2-WPRE

Plasmid

#240056

Purpose

Cre dependent Green fluorescent, negative response-polarity voltage indicator for mammalian expression

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

Ace-mNeon2

Use

AAV

Promoter

Ef1a

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGEMHE-Trim21-OLLAS

Plasmid

#237438

Purpose

Expresses mouse Trim21 ubiquitin ligase tagged with OLLAS at C-term; made for in vitro transcription (T7 promoter)

Depositor

Michael Lampson

Article

Dudka et al Nature. 2025 Feb;638(8051):814-822. doi:

Insert

Trim21 (Trim21 Mouse)

Tags

OLLAS

Expression

Mammalian

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_EGFP-BRD4-NUT-KS-FtoG

Plasmid

#238256

Purpose

For overexpression of EGFP-BRD4-NUT-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-BRD4-NUT-KS-FtoG

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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mscv_EGFP-IRES-NUP98-KDM5A-KS

Plasmid

#238290

Purpose

For overexpression of EGFP-IRES-NUP98-KDM5A-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-IRES-NUP98-KDM5A-KS

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-YAP-MAML2-KS

Plasmid

#238276

Purpose

For overexpression of mEGFP-YAP-MAML2-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mEGFP-YAP-MAML2-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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mscv_EGFP-IRES-NUP98-KDM5A

Plasmid

#238289

Purpose

For overexpression of EGFP-IRES-NUP98-KDM5A

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-IRES-NUP98-KDM5A

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_EGFP-BRD4-NUT-KS

Plasmid

#238255

Purpose

For overexpression of EGFP-BRD4-NUT-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-BRD4-NUT-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_VHH05-EGFP-NPM1

Plasmid

#238268

Purpose

For overexpression of VHH05-EGFP-NPM1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

VHH05-EGFP-NPM1

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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mscv_EGFP-NUP98-KDM5A-KS-FtoA

Plasmid

#238295

Purpose

For overexpression of EGFP-NUP98-KDM5A-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-NUP98-KDM5A-KS-FtoA

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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mscv_EGFP-NUP98-KDM5A-KS

Plasmid

#238294

Purpose

For overexpression of EGFP-NUP98-KDM5A-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-NUP98-KDM5A-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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p52K-GFP-linker-KS-FtoA

Plasmid

#237687

Purpose

For overexpression of 52K-GFP-linker-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

52K-GFP-linker-KS-FtoA

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Monbr-Sox-like

Plasmid

#231530

Purpose

Expresses Monsiga brevicolis full-length Sox-like in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-like

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Chim-Salhel-I-K57E

Plasmid

#231519

Purpose

Expresses Salpingoeca helianthica chimeric Sox-I mutant in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Salhel-I HMG K57E flanked with N- and C-termini of mSox2

Use

Lentiviral

Mutation

Point mutation at position 57 K to E of the HMG

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Salro-Sox-like

Plasmid

#231531

Purpose

Expresses Salpingoeca rosetta full-length Sox-like in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-like

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pUt-MONARCH 1.0_crEGFP

Plasmid

#224790

Purpose

LPUtopia matching RMCE donor plasmid with MONARCH 1.0 circuit with crRNA targeting EGFP. Use BlastR for positive and HSV-TK for negative selection

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

crEGFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV-d2i

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pUt-MONARCH 2.0_crEGFP

Plasmid

#224793

Purpose

LPUtopia matching RMCE donor plasmid with MONARCH 2.0 circuit with crRNA targeting EGFP.

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Inserts

crEGFP
Triplex stablier

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV-d2i

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-2O-crBFP_EF1a-BFP

Plasmid

#224783

Purpose

BFP-targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and target BFP protein expressed from EF1a promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

crBFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6-2xTetO

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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