We narrowed to 88,932 results for: Mal;

1. pVH19-Tier1-PhCMV-p300core

   Plasmid

   #169572

   Purpose

   Tier-1 vector encoding PhCMV-driven histone acetyltransferase p300 core domain expression (PhCMV-p300core-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P300core (EP300 Human)
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS2304-Tier1-Tactb-PPGK-NLuc_TB

   Plasmid

   #169549

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   Tactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS1046-Tier1-PhCMV-4xCox8-mRuby2

   Plasmid

   #169537

   Purpose

   Tier-1 vector encoding PhCMV-driven mRuby2 localized to the mitochondrial matrix via a four-fold Cox8 localization signal (PhCMV-4xCox8-mRuby2-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven miochondria-localized monomeric red fluorescent protein mRuby2
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS2380-Tier1-PhCMV-TM

   Plasmid

   #169485

   Purpose

   Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) (PhCMV-TM-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven Junctophilin 2 transmembrane domain (JPH2 Human)
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. attB53-Pac-tetO-mCherry-attB53

   Plasmid

   #183612

   Purpose

   RMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   tetO-mCherry
   

   Use

   Rmce

   Promoter

   tetO

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-HypaCas9-mR2-CfANLN_sgRNA

   Plasmid

   #183879

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   Canis familiaris ANLN sgRNA spacer
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459-HypaCas9-H2BC11_sgRNA

   Plasmid

   #183883

   Purpose

   pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 sgRNA spacer (H2BC11 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459-HypaCas9-MYH10_sgRNA

   Plasmid

   #183887

   Purpose

   pX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   MYH10 sgRNA spacer (MYH10 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-HypaCas9-mR2-H2BC11_sgRNA

   Plasmid

   #183881

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 sgRNA spacer (H2BC11 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-HypaCas9-mR2-MYH10_sgRNA

    Plasmid

    #183886

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    MYH10 sgRNA spacer (MYH10 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-hPPP3R2

    Plasmid

    #179163

    Purpose

    Expression vector of human protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2), CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-mPpp3r2

    Plasmid

    #179161

    Purpose

    Expression vector of mouse protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2), CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiCRISPRv1_COX4

    Plasmid

    #177983

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting COX4

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting COX4
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV+ (mScarlet-I) plasmid with onboard mCitrine cassette

    Plasmid

    #169743

    Purpose

    CMV+ circuit plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    mScarlet-I
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pClgn1.1_Kctd19-3xHA

    Plasmid

    #173497

    Purpose

    Express Kctd19-3xHA under the mouse Clgn promoter.

    Depositor

    Masahito Ikawa

    Article

    Oura et al PLoS Genet. 2021 May 7;17(5):e1009412. d

    Insert

    Potassium Channel Tetramerization Domain Containing 19 (Kctd19 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pClgn1.1_3xFLAG-Kctd19

    Plasmid

    #173498

    Purpose

    Express 3xFLAG-Kctd19 under the mouse Clgn promoter.

    Depositor

    Masahito Ikawa

    Article

    Oura et al PLoS Genet. 2021 May 7;17(5):e1009412. d

    Insert

    Potassium Channel Tetramerization Domain Containing 19 (Kctd19 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Equalizer-M plasmid with onboard mCherry cassette

    Plasmid

    #169734

    Purpose

    Equalizer-M plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJM573 ZF6x6-C 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA in TUPV2

    Plasmid

    #161560

    Purpose

    Inducible expression of 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA under the ZF6x6-C promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG and HA

    Expression

    Mammalian

    Promoter

    ZF6x6-C

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJM576 ZF2x6-C 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA in TUPV2

    Plasmid

    #161562

    Purpose

    Inducible expression of 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA under the ZF2x6-C promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-intC-ZF1-NLS-HA
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG and HA

    Expression

    Mammalian

    Promoter

    ZF2x6-C

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJM591 EF1α 3xFLAG-NLS-VP64-ZF1-intN in TUPV2

    Plasmid

    #161567

    Purpose

    Constitutive expression of 3xFLAG-NLS-VP64-ZF1-intN under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-ZF1-intN
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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