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pET19b-hMED9

Plasmid

#15365

Depositor

Joan Conaway, Ronald Conaway

Article

Paoletti et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

Insert

hMED9 (MED9 Human)

Tags

His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
AcrIIC1X

Plasmid

#128112

Purpose

AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrX (AcrIIC1 N3F/D15Q/A48I)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMflT-o4

Plasmid

#101312

Purpose

Contains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Insert

oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-HsSmg6-del36-576-siRNAres_I

Plasmid

#146553

Purpose

Mammalian Expression of HsSmg6-del36-576-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6-del36-576-siRNAres (SMG6 Human)

Expression

Mammalian

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP

Plasmid

#127363

Purpose

Binary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Tags

GS linker, NES, V5, and YFP

Expression

Plant

Mutation

R118G

Promoter

Arabidopsis UBQ10 promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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DYNLL1

Plasmid

#132530

Purpose

Human LC8-1, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLL1 (DYNLL1 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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DYNC2LI1

Plasmid

#132527

Purpose

Human LIC3, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNC2LI1 (DYNC2LI1 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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DYNLRB1

Plasmid

#132533

Purpose

Human Rbl-1, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLRB1 (DYNLRB1 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSR06

Plasmid

#69152

Purpose

read-outloxN mCherry to GFP switch for integration on ttTi5605, Mos Chr II

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0 and codon-optimzed index…

Promoter

rps-27

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pVL94 - pOp4:VND7

Plasmid

#116016

Purpose

destination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-induction

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR

Expression

Plant

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-EKAR

Plasmid

#181858

Purpose

Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-EKAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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p6900 PHAGE-P CMVT N-HA-hE6AP III C840A

Plasmid

#37606

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP III C840A (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C840A

Promoter

CMV tet regulatable

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
Venus-G protein Gamma 5

Plasmid

#42200

Depositor

Narasimhan Gautam

Article

Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

Insert

Gamma 5 (Gng5 Rat)

Tags

Venus(1-155)

Expression

Mammalian

Mutation

codon-optimized

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
psiCHECK IRF9 3'UTR

Plasmid

#19863

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

IRF9 3'UTR (IRF9 Human)

Expression

Mammalian

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HRPT2 L64P

Plasmid

#11052

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 L64P (CDC73 Human)

Tags

Flag

Expression

Mammalian

Mutation

L64P

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
vsv-CenpB 1-166-Sgo1 N61I-GFP

Plasmid

#108499

Purpose

expression of vsv-CenpB 1-166-Sgo1 N61I-EGFP (no PP2A binding)

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

CenpB-Shugoshin 1 (SGO1 Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CenpB 1-166 fusion before Sgo1, no PP2A binding N…

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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phumanCas9 (GB0575)

Plasmid

#68206

Purpose

Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Cas9 coding region (human codon optimised)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed; human codon optimis…

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS DNAJA4

Plasmid

#19548

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily A, member 4 (DNAJA4 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pJZC39

Plasmid

#62333

Purpose

sgRNA + 1x PP7 with mCherry for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x PP7
mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_PSTPIP1-1/1

Plasmid

#91453

Purpose

Protein expression and purification of human SH3 domain construct PSTPIP1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PSTPIP1-1/1 (PSTPIP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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