We narrowed to 19,676 results for: Cre

1. Venus-7aa-ActA-pEGFP-C1

   Plasmid

   #177405

   Purpose

   To express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.

   Depositor

   David Andrews

   Article

   Osterlund et al Protocol Exchange (2022)

   Insert

   ActA tail anchor (actA Listeria monocytogenes)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-REPORT(PGK)

   Plasmid

   #172328

   Purpose

   LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

   Plasmid

   #105039

   Purpose

   Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Tsc2 (Tsc2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. LB124 (Bcl-2 from -1287 to -3934)

   Plasmid

   #15240

   Depositor

   Linda Boxer

   Article

   Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

   Insert

   B-cell lymphoma/leukemia-2 (BCL2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Bacterial

   Mutation

   Bcl-2 fragment from -1287 to -3934 with P1

   Available Since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pRS416 Gal G294A YFP

   Plasmid

   #27448

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Glycine 294 mutated to Alanine (G294A)

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

6. KM-CashGem-Xyl2-URA3-LINEAR

   Plasmid

   #174838

   Purpose

   pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Promoter

   TEF1p

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_APOBEC1-UdgX-nCas9

   Plasmid

   #163531

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   APOBEC1-UdgX-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiCRISPRv2puro-Tbxas1

   Plasmid

   #170305

   Purpose

   A knock-out vector for the mouse Tbxas1

   Depositor

   Michiyuki Matsuda

   Article

   Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

   Insert

   A gRNA targeting the mouse Tbxas1 gene.
   

   Use

   CRISPR and Lentiviral

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pINDUCER21-puro_TAOK2

   Plasmid

   #172979

   Purpose

   doxycycline-inducible expression of TAOK2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   TAOK2 (TAOK2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-ING1a

    Plasmid

    #79069

    Purpose

    Expression of p47ING1 in mammalian cells

    Depositor

    Karl Riabowol

    Article

    Soliman et al Aging Cell. 2008 Dec;7(6):783-94. doi: 1

    Insert

    ING1 (p47 ING1A isoform) (ING1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pLJC6-GPT1-3xFLAG

    Plasmid

    #163448

    Purpose

    lentiviral overexpression vector for human GPT1

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    GPT1 (GPT Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    See: comments

    Promoter

    Ubc

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Sema3e(L)-Fc-His

    Plasmid

    #72145

    Purpose

    Expresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3e (Sema3e Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. phFluc-WT

    Plasmid

    #177714

    Purpose

    Cytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019

    Depositor

    Erik Böttger

    Article

    Shcherbakov et al Commun Biol. 2019 Oct 17;2:381. doi: 10.

    Insert

    hFluc WT
    

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. IG510: pMVP (R4-R3) human PDX1

    Plasmid

    #121744

    Purpose

    pMVP R4-R3 entry plasmid, contains human PDX1 for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Human PDX1 (PDX1 Human)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a_NEUROG1_P2A_Hygro_Barcode

    Plasmid

    #120467

    Purpose

    Barcoded lentiviral vector to express NEUROG1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    NEUROG1 (NEUROG1 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pD2529-CAG-aIIb

    Plasmid

    #189796

    Purpose

    Expression of full length human integrin aIIb

    Depositor

    Timothy Springer

    Article

    Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

    Insert

    aIIb (ITGA2B Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pECE-M2-PAK2 S20A

    Plasmid

    #31681

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    p21-activated protein kinase 2 S20A (PAK2 Human)
    

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Serine 20 to Alanine

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. LifeAct-mAvicFP1

    Plasmid

    #129512

    Purpose

    Expresses LifeAct-mAvicFP1 in mammalian cells

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    LifeAct-mAvicFP1
    

    Expression

    Mammalian

    Mutation

    mAvicFP1 is AvicFP1 F64L + A206K

    Promoter

    CMV

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. DCT.VV.Orf40

    Plasmid

    #12728

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf40
    

    Expression

    Mammalian

    Available Since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

20. Sema4a-Fc-His

    Plasmid

    #72154

    Purpose

    Expresses the extracellular region of the Sema4A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4a (Sema4a Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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