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pET30_Halotag_K73T_Jdomain

Plasmid

#183693

Purpose

Bacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domain

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(K73T)-J domain

Expression

Bacterial

Mutation

K73T in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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p2621

Plasmid

#198144

Purpose

Epitope tagging of effector protein PipB2

Depositor

Michael Hensel

Article

Goser et al Nat Commun. 2023 Mar 4;14(1):1240. doi:

Insert

PpipB2 pipB2::M45 (STM2780 Salmonella enterica serovar Typhimurium)

Tags

M45

Expression

Bacterial

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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p2643

Plasmid

#198145

Purpose

Epitope tagging of effector protein SseF

Depositor

Michael Hensel

Article

Goser et al Nat Commun. 2023 Mar 4;14(1):1240. doi:

Insert

PsseA::sscB sseF::HA (sseF Salmonella enterica serovar Typhimurium)

Tags

HA

Expression

Bacterial

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-HsSmg6-mut-siRNAres_I

Plasmid

#146588

Purpose

Mammalian Expression of HsSmg6-mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

Insert

HsSmg6-mut-siRNAres (SMG6 Human)

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pORE303N_CEN12Fuzzy3MYB

Plasmid

#194440

Purpose

CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12 and Fuzzy3MYB

Depositor

Chung-Jui Tsai

Article

Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

Insert

gRNA array targeting CEN1, Fuzzy3MYB

Use

CRISPR

Expression

Plant

Promoter

MtU6

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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HySp5-310

Plasmid

#192995

Purpose

Plasmid that encodes for Hydra Sp5, lacking the SP box

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Tags

HA

Expression

Mammalian

Mutation

no SP box

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pORE303N_CEN1

Plasmid

#194439

Purpose

CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout CEN1

Depositor

Chung-Jui Tsai

Article

Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

Insert

gRNA targeting CEN1

Use

CRISPR

Expression

Plant

Promoter

MtU6

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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OA-1093B

Plasmid

#194003

Purpose

pBac-U6-crCHIKV-array-UTR-3xp3-tdTomato

Depositor

Omar Akbari

Article

Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

Insert

4 gRNAs targeting CHIKV

Expression

Insect

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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OA-1085F

Plasmid

#191375

Purpose

pBac-U6-crYellow-array-UTR-3xp3-tdTomato

Depositor

Omar Akbari

Article

Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

Insert

4 gRNAs targeting yellow

Expression

Insect

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC2

Plasmid

#191857

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003_sgTFAP4 guide 2

Plasmid

#193587

Purpose

TFAP4 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgTFAP4 guide 2 (TFAP4 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC3

Plasmid

#191858

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-T-Easy-HySp5-557

Plasmid

#193000

Purpose

Plasmid to generate an ISH probe for Hydra Sp5

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Use

Cloning vector

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ZfSp5a-377

Plasmid

#192997

Purpose

Plasmid that encodes for Zebrafish Sp5

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Zebrafish Sp5

Tags

HA

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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HySp5-227

Plasmid

#192996

Purpose

Plasmid that encodes for Hydra Sp5, lacking the SP box and DNA binding domain

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Tags

HA

Expression

Mammalian

Mutation

no SP box and DNA binding domain

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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HySp5:Luc

Plasmid

#192985

Purpose

Plasmid to test the Hydra Sp5 promoter activity

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5 promoter

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ZfSp5l1

Plasmid

#192999

Purpose

Plasmid that encodes for Zebrafish Sp5-like

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Zebrafish Sp5-like

Tags

HA

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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HySp5-337

Plasmid

#192994

Purpose

Plasmid that encodes for Hydra Sp5, lacking the DNA binding domain

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Tags

HA

Expression

Mammalian

Mutation

no DNA binding domain

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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ZfWnt3:Luc

Plasmid

#192992

Purpose

Plasmid to test the Zebrafish Wnt3 promoter activity

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Zebrafish Wnt3 promoter

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Gly-tRNA-gRNA scaffold for library 2

Plasmid

#192365

Purpose

tRNA sequence provider (for checkpoint library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Gly tRNA-gRNA scaffold

Use

For golden gate assembly

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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