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pJBL7079

Plasmid

#167244

Purpose

cell-free lac sensor with GFP output

Depositor

Julius Lucks

Publication

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pLac-sfGFP

Use

Synthetic Biology

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7080

Plasmid

#167245

Purpose

cell-free lac sensor with RFP output

Depositor

Julius Lucks

Publication

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pLac-mRFP1

Use

Synthetic Biology

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7081

Plasmid

#167246

Purpose

cell-free lac sensor with Mango output

Depositor

Julius Lucks

Publication

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pLac-Mango

Use

Synthetic Biology

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7082

Plasmid

#167247

Purpose

cell-free lac sensor with XylE output

Depositor

Julius Lucks

Publication

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pLac-XylE

Use

Synthetic Biology

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7084

Plasmid

#167248

Purpose

overexpression of LacI

Depositor

Julius Lucks

Publication

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pT7-LacI

Expression

Bacterial

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7086

Plasmid

#167250

Purpose

orthogonal Mango reporter output for cell-free expression

Depositor

Julius Lucks

Publication

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pAKSIRV-Mango

Use

Synthetic Biology

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pL0_EF-H4

Plasmid

#202178

Purpose

Level 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

H4 terminator

Use

Synthetic Biology

Mutation

None

Available since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-H4

Plasmid

#202122

Purpose

Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

H4 promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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L_NLS 27kDa

Plasmid

#201365

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A4-EGFP

Expression

Mammalian

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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SP_H6_Halo_K73T_KDEL_pBABEpu

Plasmid

#183689

Purpose

Retroviral expression of ER-targeted metastable Halotag/folding sensor (HaloK73T)

Depositor

Edward Avezov

Publication

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(K73T)

Use

Retroviral

Mutation

K73T in Halotag

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_ChR2-3M-YFP

Plasmid

#186977

Purpose

Expresses ChR2-3xM channelrhodopsin in mammalian cells

Depositor

Adam Cohen

Publication

Hayward et al eLife. 2023 Oct 6;12:RP86833. doi: 10.75

Insert

ChR2-3xM-YFP

Use

Lentiviral

Tags

eYFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-18 TC-sfGFP

Plasmid

#194632

Purpose

fluorescent protein with tetracysteine tag

Depositor

Allie Obermeyer

Publication

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

tetracysteine-sfGFP

Tags

MGCCPGCCGGA

Expression

Bacterial

Promoter

T7

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEY18

Plasmid

#191024

Purpose

flp-19 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-19 (flp-19 Nematode)

Expression

Worm

Mutation

None

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY29

Plasmid

#191034

Purpose

gcy-36 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-36 (gcy-36 Nematode)

Expression

Worm

Mutation

None

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY64

Plasmid

#191061

Purpose

aqp-6 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

aqp-6 (aqp-6 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY49

Plasmid

#191053

Purpose

lim-6 fluorescent neural reporter driving nuclear mNeptune2.5 and CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

lim-6 (lim-6 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAP01

Plasmid

#186260

Purpose

sfGFP under light light responsive PEL222 promoter

Depositor

Yongku Cho

Publication

Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

Insert

sfGFP

Expression

Bacterial

Promoter

PEL222 (GGTAGCCTTTAGTCCATGTTAGCGAAGAAAATGGTTTGTTA…

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA iRFP713_zhang2.0

Plasmid

#167916

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Puro_zhang2.0

Plasmid

#167919

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Blasto_zhang2.0

Plasmid

#167912

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge