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lvl2-[piggyBac-backbone]

Plasmid

#229209

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-2_M13ps

Plasmid

#231412

Purpose

M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-2

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A1_M13ps

Plasmid

#231414

Purpose

M13 phagemid that expresses sgRNA-A1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ164.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-A1

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHACK-Sparse.FRT10-GAL4.DBD

Plasmid

#232833

Purpose

Sparse.FRT10 GAL4.DBD for HACK system

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10
GAL4.DBD (GAL4 Budding Yeast)

Use

CRISPR

Expression

Insect

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBP-Sparse.FRT10-GAL4.DBD

Plasmid

#232831

Purpose

Enhancer-free Sparse.FRT10 GAL4.DBD with ccdB for Gateway

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10
GAL4.DBD (GAL4 Budding Yeast)

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-S1S2 [backbone]

Plasmid

#229194

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl2-[lenti-backbone]

Plasmid

#229208

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
lvl0-P [backbone]

Plasmid

#229154

Purpose

Promoter backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
lvl0-5 [backbone]

Plasmid

#229155

Purpose

5 prime backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
lvl0-G [backbone]

Plasmid

#229156

Purpose

Gene backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
lvl0-3 [backbone]

Plasmid

#229157

Purpose

3 prime backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
lvl0-T [backbone]

Plasmid

#229158

Purpose

Terminator backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pJL1-sFLAG-p5-PRB4_R7-9-FETCH1

Plasmid

#210651

Purpose

In vitro expression of polyProline FETCH reporter from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

sFLAG-p5-PRB4_R7-9-FETCH1

Use

Synthetic Biology

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJL1-sFLAG-p1-PRB4_R7-9-FETCH1

Plasmid

#210652

Purpose

In vitro expression of polyProline FETCH reporter from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

sFLAG-p1-PRB4_R7-9-FETCH1

Use

Synthetic Biology

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJL1-sFLAG-a5-PRB4_R7-9A-FETCH1

Plasmid

#210653

Purpose

In vitro expression of polyProline FETCH control from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

sFLAG-a5-PRB4_R7-9A-FETCH1

Use

Synthetic Biology

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJL1-sFLAG-FETCH1

Plasmid

#210646

Purpose

In vitro expression of FETCH reporter from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

FETCH1

Use

Synthetic Biology

Tags

superFLAG (DYKDEDLL)

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJL1-sFLAG-FETCH2

Plasmid

#210647

Purpose

In vitro expression of FETCH reporter from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

FETCH2

Use

Synthetic Biology

Tags

superFLAG (DYKDEDLL)

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJL1-FETCH2-StrepII

Plasmid

#210649

Purpose

In vitro expression of FETCH reporter from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

FETCH2

Use

Synthetic Biology

Tags

Strep-TagII

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-ChmF

Plasmid

#221345

Purpose

Express ChmF in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmF

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-ChmK

Plasmid

#221346

Purpose

Express ChmK in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmK

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge