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pET22-HT/prsA

Plasmid

#73624

Purpose

Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

ribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GFP-TAG

Plasmid

#158131

Purpose

Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

sfGFP

Expression

Yeast

Mutation

Introduced a stop codon in place of the tyrosine …

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-HOXA1

Plasmid

#47054

Purpose

Homeobox A1

Depositor

Huda Zoghbi

Article

Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

Insert

Homeobox A1 (HOXA1 Human)

Use

Gateway Cloning

Available Since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pRS416-Aga1p-FAPB2.3.6L1TAG

Plasmid

#158148

Purpose

Yeast display reporter for non-display yeast strains (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p-FAPB2.3.6

Expression

Yeast

Mutation

Cloned in a TAG codon at the first position of th…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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MT21-Ubc9

Plasmid

#17273

Depositor

Stephen Goff

Article

Yueh et al J Virol. 2006 Jan . 80(1):342-52.

Insert

Ubc9 (Ube2i Mouse)

Expression

Mammalian

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
pMSCV-PIG-miR-30b

Plasmid

#64232

Purpose

Expresses miR-30b in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-30b (MIR30B Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pOC1 Gria1-GFP-FRB KI

Plasmid

#183428

Purpose

CreOFF knock-in for GluA1-GFP-FRB (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP-FRB donor

Use

CRISPR

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Dlg4-Halo-FKBP KI

Plasmid

#183435

Purpose

CreOFF knock-in for PSD95-Halo-FKBP (amino acid position: R721)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo-FKBP donor

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG TBC1D13

Plasmid

#87953

Purpose

Expresses flag tagged mouse TBC1D13

Depositor

David James

Article

Davey et al Traffic. 2012 Oct;13(10):1429-41. doi: 1

Insert

TBC1D13 (Tbc1d13 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pOC3 smFP-HA-Cacna1e KI

Plasmid

#183436

Purpose

CreON FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP HA donor

Use

CRISPR

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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