We narrowed to 4,716 results for: golden gate

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sgPlxnb2-1 in pT4-U6-sgRNA-CMV-EGFP

Plasmid

#239588

Purpose

expresses guide#1 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

sgRNA targeting TSS-upstream reagion of Plxnb2 (Plxnb2 Mouse)

Use

Sleeping beauty (sb) transposon

Expression

Mammalian

Promoter

hU6

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hUbC-EGFP-sgRNA.FOXA1/A2/A3_CRISPRi

Plasmid

#216166

Purpose

Express the gRNAs for the human FOXA1/A2/A3-CRISPRi

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA-FOXA1-Rank1, sgRNA-FOXA2-Rank2, sgRNA-FOXA2-Rank1, and sgRNA-FOXA3-Rank1 (FOXA1, FOXA2, FOXA3 Human)

Use

Lentiviral

Tags

EGFP

Promoter

phU6, ph7SK, phH1, pmU6

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pML390_YWHAQ-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227925

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

YWHAQ homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML389_YWHAB-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227926

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

YWHAB homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML357_CAV1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227914

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CAV1 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML361_DCP1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227918

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

DCP1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML366_HSPA1B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227920

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

HSPA1B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML365_HSP90AA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227921

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

HSP90AA homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML364_PSMB7-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227922

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

PSMB7 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML374_CLTA-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227893

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CLTA homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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