Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 9,921 results for: pET

Showing: 9341 - 9360 of 9921 results

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M53

Plasmid

#242621

Purpose

Strep-Flag-MYCMutMBII-His protein expression in bacteria

Depositor

Alain Nepveu

Publication

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

Strep-Flag-MYCMutMBII-His (MYC Human)

Tags

His and Strep

Expression

Bacterial

Mutation

I128A,I129S,I130A,M134A,F137A

Available since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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M24

Plasmid

#242619

Purpose

Strep- MYC deltaCTD, deltaMB0-1-His protein expression in bacteria

Depositor

Alain Nepveu

Publication

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

Strep- MYC deltaCTD, deltaMB0-1-His (MYC Human)

Tags

His and Strep

Expression

Bacterial

Mutation

delta2-62, delta354-439

Available since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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M101

Plasmid

#242624

Purpose

Strep-MYC-MutBoxIIIb-His protein expression in bacteria

Depositor

Alain Nepveu

Publication

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

Strep-MYC-MutBoxIIIb-His (MYC Human)

Tags

His and Strep

Expression

Bacterial

Mutation

E259A,E260K,E261S,D263S,E268A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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His6-tev-G(TTR A25T)

Plasmid

#238116

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Publication

Sun et al J Am Chem Soc. 2024 Jan 10;146(1):532-54

Insert

TTR (TTR Human)

Tags

His6tev-G

Expression

Bacterial

Mutation

A25T

Promoter

T7

Available since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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TTR F87E

Plasmid

#238075

Purpose

Bacterial expression of human TTR mutants

Depositor

Peter Wright

Publication

Leach et al J Mol Biol. 2024 Aug 15;436(16):168673.

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

F87E

Promoter

T7

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WT 44merHA

Plasmid

#232988

Purpose

Galactose iduced expression of Gcn4 WT 44merHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WT 44mer

Tags

3xHA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 L123A

Plasmid

#232969

Purpose

Galactose iduced expression of Gcn4 L123A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L123A

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123A

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Swap2

Plasmid

#232965

Purpose

Galactose iduced expression of Gcn4 k0.19 in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 k0.19

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Swap3

Plasmid

#232960

Purpose

Galactose iduced expression of Gcn4 k0.47 in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 k0.47

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 E+

Plasmid

#232963

Purpose

Galactose iduced expression of Gcn4 E+in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E+

Tags

TEV cleavage site

Expression

Yeast

Mutation

D105E, D118E, D139E

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 W120A

Plasmid

#232962

Purpose

Galactose iduced expression of Gcn4 W120A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 W120A

Tags

TEV cleavage site

Expression

Yeast

Mutation

W120A

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 R3

Plasmid

#232973

Purpose

Galactose iduced expression of Gcn4 R++ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R++

Tags

TEV cleavage site

Expression

Yeast

Mutation

D103R, D133R, K143R

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Swap3GFP

Plasmid

#233010

Purpose

Galactose iduced expression of Gcn4 k0.47GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 k0.47

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 F124I

Plasmid

#232964

Purpose

Galactose iduced expression of Gcn4 F124I in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F124I

Tags

TEV cleavage site

Expression

Yeast

Mutation

F124I

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Swap3HA

Plasmid

#232998

Purpose

Galactose iduced expression of Gcn4 k0.47HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 k0.47

Tags

3xHA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WT 44mer

Plasmid

#232956

Purpose

Galactose iduced expression of Gcn4 WT 44mer in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WT 44mer

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WT 44merGFP

Plasmid

#233000

Purpose

Galactose iduced expression of Gcn4 WT 44merGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WT 44mer

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WT 30mer

Plasmid

#232986

Purpose

Galactose iduced expression of Gcn4 WT 30mer in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WT 30mer

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 S/ArotoL

Plasmid

#231880

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 SArotoL

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 S/ArotoL

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101L, S104L, F108L, Y110L, S117L, S136L, S144L

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAIP4_4J5Q

Plasmid

#234233

Purpose

Heterologous protein expression of ADP-ribose glycohydrolase OARD1 in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

4J5Q (tas3 Human)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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