We narrowed to 17,098 results for: IGH@

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Dom34 K45A

Plasmid

#19111

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

C tag

Expression

Bacterial

Mutation

K45A

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pRK5_tAKARδ

Plasmid

#119916

Purpose

Mammalian expression of the tAKARδ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARδ

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARε

Plasmid

#119917

Purpose

Mammalian expression of the tAKARε sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARε

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pLIB-HISX6-TEV-RSP4

Plasmid

#176693

Purpose

Expression of Chlamydomonas reinhardtii RSP4 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP4

Tags

HISX6

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

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pLIB-RSP5

Plasmid

#176694

Purpose

Expression of Chlamydomonas reinhardtii RSP5 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP5 (RSP5 Chlamydomonas reinhardtii)

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

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pBR9-genomic_RSP6-HAX3

Plasmid

#176703

Purpose

Rescue of RSP6 Chlamydomonas mutant with tagged RSP6

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP6

Tags

HAX3

Expression

Plant

Mutation

None

Promoter

rbcs2

Availability

Academic Institutions and Nonprofits only

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dCAS9-VP64_GFP

Plasmid

#61422

Purpose

Expresses dCAS9-VP64 activator with 2A GFP

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Concentrated Lentiviral Prep

Insert

dCAS9(D10A,H840A)-VP64_2A_GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

EF1A

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP (PX458)

Plasmid

#48138

Purpose

Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Has Service

Cloning Grade DNA

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCRISPRia-v2

Plasmid

#84832

Purpose

CRISPRi/a V2 library parental plasmid

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Inserts

sgRNA
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

Plasmid

#79671

Purpose

Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

Depositor

Scott Sternson

Article

Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

Insert

EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-iLACCO1

Plasmid

#218821

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1

Expression

Mammalian

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMD2-VSVGmut

Plasmid

#182229

Purpose

Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

VSVGmut

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pREDTAI

Plasmid

#51627

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

trc

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0

Plasmid

#62987

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-G-Flamp1

Plasmid

#188567

Purpose

green biosensor for cAMP in mammalian cells

Depositor

Jun Chu

Article

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1

Tags

His tag

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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BA31_pU.CAG.SaCas9-D10A.rBGpA

Plasmid

#199251

Purpose

Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

SaCas9.D10A nickase

Use

CRISPR

Tags

3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAGM65879

Plasmid

#153214

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and Bar selection cassette

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-mNeongreen

Plasmid

#182389

Purpose

Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB502

Plasmid

#74844

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-iLACCO1

Plasmid

#218817

Purpose

Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1

Expression

Bacterial

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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