We narrowed to 12,028 results for: LEA
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Plasmid#231873PurposeBacterial expression of N-terminally 6His tagged Gcn4 L113A+DepositorInsertGcn4 L113A+
Tags6xHis, TEV cleavage siteExpressionBacterialMutationL113A, E114Y, N126H, D127E, S136APromoterT7Available SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 Aro3+
Plasmid#231884PurposeBacterial expression of N-terminally 6His tagged Gcn4 Aro3+DepositorInsertGcn4 Aro3+
Tags6xHis, TEV cleavage siteExpressionBacterialMutationD103Y, N112T, L113Y, S117F, K140RPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 Aro2+
Plasmid#231883PurposeBacterial expression of N-terminally 6His tagged Gcn4 Aro2+DepositorInsertGcn4 Aro2+
Tags6xHis, TEV cleavage siteExpressionBacterialMutationN116F, S117E, E119N, P129Q, T132YPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Cdk13_intron3_sg4_pX458
Plasmid#127339PurposesgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #4DepositorAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTarget-Dual
Plasmid#207884PurposeCarries the cognate target sites for the homing endonucleases I-OnuI and I-GpeMI.DepositorInsertsI-OnuI target site
I-GpeMI target site
UseSynthetic BiologyExpressionBacterialAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-PCi
Plasmid#214038PurposeBacterial expression plasmid for strep-tagged Pci from Haliangium ochraceumDepositorInsertPCi
TagsStrep-tag IIExpressionBacterialMutationWTPromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAVED-CHAT-sm_PCaspase
Plasmid#214048PurposeBacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceumDepositorInsertSAVED-CHAT-PCaspase
ExpressionBacterialMutationR456E, D459K, D476K, R479EPromoterlacUV5 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-SAVED-CHAT
Plasmid#214030PurposeBacterial expression plasmid for strep-tagged wild-type SAVED-CHAT from Haliangium ochraceumDepositorInsertSAVED-CHAT
TagsStrep-tag IIExpressionBacterialMutationWTPromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-PC-σ
Plasmid#214037PurposeBacterial expression plasmid for strep-tagged PC-σ from Haliangium ochraceumDepositorInsertPC-σ
TagsStrep-tag IIExpressionBacterialMutationWTPromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxEXPr6
Plasmid#218290PurposeIntroduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3DepositorInserthph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1314
Plasmid#217956PurposeExpression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H3.1 (H3C1 Human)
TagseGFPExpressionMammalianMutationGGG insertion between A95 and C96PromoterCMVAvailable SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-human-pro-IL-18_V47N_I48N
Plasmid#214319PurposeExpression of gene in mammalian cells by retroviral transductionDepositorAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-human-pro-IL-18_LES33AAA
Plasmid#214321PurposeExpression of gene in mammalian cells by retroviral transductionDepositorAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-human-pro-IL-18_DDEN29ENGD
Plasmid#214320PurposeExpression of gene in mammalian cells by retroviral transductionDepositorAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-human-pro-IL-18_E192K_D193K
Plasmid#214318PurposeExpression of gene in mammalian cells by retroviral transductionDepositorAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_StammerAEA
Plasmid#202588PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purificationDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Methionine 91 to Alanine, changed O…Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_CRF0.5
Plasmid#208657PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in neuronsDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
UseAAVPromoterhSynAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2A
Plasmid#195710PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2A domainDepositorInsertSyt1 (Syt1 Rat)
TagsGSTExpressionBacterialMutationencodes amino acids 96-264 of mouse SYT1Available SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2AB
Plasmid#195712PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2AB domainDepositorInsertSyt1 (Syt1 Rat)
TagsGSTExpressionBacterialMutationencodes amino acids 96-421 of mouse SYT1Available SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOET3-C-TEV-Twin-Strep
Plasmid#203324PurposeGenerate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cellsDepositorTypeEmpty backboneTagsTEV cleavable site (ENLYFQG) and Twin-strep-tagExpressionInsectAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only