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Showing: 1841 - 1880 of 5866 results

  2. FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP

    Plasmid
    #194890
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_K365A_R368A
    U6-crRNA1
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    194890-plasmid-map-sequence-id-392583

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  3. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP

    Plasmid
    #194895
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_Q418A
    U6-crRNA2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    April 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194895-plasmid-map-sequence-id-389971

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  4. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP

    Plasmid
    #194894
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_K365A_R368A
    U6-crRNA2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    194894-plasmid-map-sequence-id-382875

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  5. FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

    Plasmid
    #194889
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-1 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_WT
    U6-crRNA1
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    194889-plasmid-map-sequence-id-382866

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  6. FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP

    Plasmid
    #194891
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-1 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_Q418A
    U6-crRNA1
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    194891-plasmid-map-sequence-id-382865

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  7. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_R396A-HA-P2A-tagBFP

    Plasmid
    #194896
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-R396A mutant and crRNA-2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_R396A
    U6-crRNA2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    194896-plasmid-map-sequence-id-382861

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  8. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_R396A-HA-P2A-tagBFP

    Plasmid
    #194888
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-R396A mutant and crRNA-1 and -2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_R396A
    U6-crRNA1and2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    194888-plasmid-map-sequence-id-382857

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  9. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP

    Plasmid
    #194886
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 and -2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_K365A_R368A
    U6-crRNA1and2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    194886-plasmid-map-sequence-id-382823

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  10. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP

    Plasmid
    #194887
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-1 and -2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_Q418A
    U6-crRNA1and2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    194887-plasmid-map-sequence-id-382813

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  11. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

    Plasmid
    #194893
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_WT
    U6-crRNA2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    194893-plasmid-map-sequence-id-382802

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  26. pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid
    #87829
    Purpose
    High-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    MTS
    NLS
    PTS1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter, tetracycline operator
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87829-plasmid-map-sequence-id-175007

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  27. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid
    #107941
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R)
    Use
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R and K548R
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107941-plasmid-map-sequence-id-418295

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  30. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

    Plasmid
    #104571
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains
    Depositor
    Keith Joung
    Article
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Insert
    human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
    Use
    CRISPR
    Tags
    NLS-3xHA-3xFLAG-4xDmrA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    104571-plasmid-map-sequence-id-201436

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  31. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

    Plasmid
    #121174
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121174-plasmid-map-sequence-id-234993

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  36. pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid
    #159857
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Article
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159857-plasmid-map-sequence-id-318022

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Showing: 1841 - 1880 of 5866 results