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Showing: 2301 - 2350 of 4184 results

  1. pJK695

    Plasmid
    #71644
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His17>Trp mutant (TdPurE2-H17W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-17 to tryptophan
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71644-plasmid-map-sequence-id-131665

    Enlarge

  2. pJK696

    Plasmid
    #71645
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met15>Trp mutant (TdPurE2-M15W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes methionine-15 to tryptophan
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71645-plasmid-map-sequence-id-131666

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  3. pJK607

    Plasmid
    #71626
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Cys mutant (TdPurE2-S38C)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to cysteine
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71626-plasmid-map-sequence-id-131645

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  4. pJK617

    Plasmid
    #71627
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Ala mutant (TdPurE2-S38A)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to alanine
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71627-plasmid-map-sequence-id-131646

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  5. pJK643

    Plasmid
    #71628
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Thr mutant (TdPurE2-S38T)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to threonine
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71628-plasmid-map-sequence-id-131647

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  6. pJK644

    Plasmid
    #71629
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp mutant (TdPurE2-S38D)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to aspartate
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71629-plasmid-map-sequence-id-131648

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  7. pJK125

    Plasmid
    #73336
    Purpose
    Encodes Mycobacterium tuberculosis FdxB (N-terminal fatty acid desaturase domain)
    Depositor
    T. J. Kappock
    Insert
    electron transfer flavoprotein
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    encodes residues 1-327
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73336-plasmid-map-sequence-id-138202

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  8. pJK553

    Plasmid
    #72452
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72452-plasmid-map-sequence-id-133889

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  9. pJK636

    Plasmid
    #72466
    Purpose
    Produces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72466-plasmid-map-sequence-id-133767

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  10. pJK530

    Plasmid
    #72511
    Purpose
    Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster with C-terminal His6 tag (AaOrfYH6)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein in purSQL operon
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72511-plasmid-map-sequence-id-132537

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  11. pJK533

    Plasmid
    #72514
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, small subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    contains additional Ala2
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72514-plasmid-map-sequence-id-135820

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  12. pJK575

    Plasmid
    #72526
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with C-terminal His6 tag (AaPurLH6)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72526-plasmid-map-sequence-id-132611

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  13. pJK620

    Plasmid
    #72537
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6-tag (H6AaPurS)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, small subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72537-plasmid-map-sequence-id-135983

    Enlarge

  14. pJK622

    Plasmid
    #72538
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with N-terminal His6-tag (H6AaPurL)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72538-plasmid-map-sequence-id-132629

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  15. pJK590

    Plasmid
    #71698
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag (AaTrxB1H6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase 1
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71698-plasmid-map-sequence-id-131731

    Enlarge

  16. pJK648

    Plasmid
    #71611
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Phe79>Trp mutant (TdPurE2-F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes phenylalanine-79 to tryptophan
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71611-plasmid-map-sequence-id-131688

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  17. 4RXJ

    Plasmid
    #64664
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WHSC1L1:JMC098-D03:C233028 (WHSC1L1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    May 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. 4JWQ

    Plasmid
    #60084
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PB000947.00.0:MAC03Z-H05:C218239
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. Py-U5-15kD

    Plasmid
    #25561
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Py-U5-15kD
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. Py-OMPDC

    Plasmid
    #25578
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Py-OMPDC (PY01515 Plasmodium yoelii)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 8, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. Py-1Cys-PRX

    Plasmid
    #25580
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Py-1Cys-PRX
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. Pv-2Cys-Prx

    Plasmid
    #25586
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pv-2Cys-Prx
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. Cp-NHP2

    Plasmid
    #25560
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Cp-NHP2 (cgd6_3850 Cryptosporidiuim parvum)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. TDRD2

    Plasmid
    #25210
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TDRD2_11 (TDRKH Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. NEDD4

    Plasmid
    #25141
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    nedd4.0001.0152 (NEDD4 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. pAIP4_4WDC

    Plasmid
    #234153
    Purpose
    Heterologous protein expression of DELTA-actitoxin-Afr1a (DELTA-AITX-Afr1a) (Fragaceatoxin C) (fraC) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4WDC
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234153-plasmid-map-sequence-id-464119

    Enlarge

  27. USP2

    Plasmid
    #36894
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP2 (USP2 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. Pf-ClpP

    Plasmid
    #25591
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pf-ClpP
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. pmSod1-WT-N’His

    Plasmid
    #117701
    Purpose
    Expression of WT mouse SOD1 with N- terminal His tag
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117701-plasmid-map-sequence-id-227503

    Enlarge

  31. USP15

    Plasmid
    #28282
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15 (USP15 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Contains dusp and Ubl domains (residues 6-223).
    Promoter
    Available since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  32. RAB1A

    Plasmid
    #25521
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB1A (RAB1A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. pmSod1-G93A-N’His

    Plasmid
    #117705
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    G93A
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117705-plasmid-map-sequence-id-227526

    Enlarge

  34. pJK407

    Plasmid
    #72421
    Purpose
    Produces Acetobacter aceti 1023 Est06, C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    lipase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    lacks Met1 and Thr2 in annotated (v1) sequence
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72421-plasmid-map-sequence-id-134675

    Enlarge

  36. pJK234

    Plasmid
    #73324
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis proteins VioA, VioB, and VioC
    Depositor
    T. J. Kappock
    Inserts
    violacein biosynthesis protein VioA
    violacein biosynthesis protein VioB
    violacein biosynthesis protein VioC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    73324-plasmid-map-sequence-id-135032

    Enlarge

  37. SUV39H2

    Plasmid
    #25115
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SUV39H2_04 (SUV39H2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. RAB5B

    Plasmid
    #25251
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB5B:A15-G191 (RAB5B Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids A15-G191
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. USP5

    Plasmid
    #25299
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP5.001.835 (USP5 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. RAB43

    Plasmid
    #25513
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB43 (RAB43 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  41. NNMT

    Plasmid
    #40734
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NNMT (NNMT Human)
    Use
    Tags
    His Thrombin
    Expression
    Bacterial
    Mutation
    K100A:E101A:E103A
    Promoter
    Available since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  42. 6NM4

    Plasmid
    #162257
    Purpose
    Bacterial Expression
    Depositor
    Cheryl Arrowsmith
    Insert
    PRDM9 (PRDM9 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162257-plasmid-map-sequence-id-319826

    Enlarge

  43. TBXTA-c006

    Plasmid
    #139755
    Purpose
    For protein expression/crystallography (PDB:6F59)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-S224
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139755-plasmid-map-sequence-id-296509

    Enlarge

  44. 5KKP (PUS7)

    Plasmid
    #101878
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PUS7 (PUS7 Human)
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101878-plasmid-map-sequence-id-194704

    Enlarge

  45. RAB14

    Plasmid
    #25519
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB14 (RAB14 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 11, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  46. PPIC

    Plasmid
    #25606
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PPIC (PPIC Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. 4MVT

    Plasmid
    #60101
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PIAS3:YTC008-B10:C225910 (PIAS3 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  48. pJK651

    Plasmid
    #71702
    Purpose
    Produces E. coli thioredoxin reductase with a C-terminal His6 tag (EcTrxBH6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71702-plasmid-map-sequence-id-131727

    Enlarge

  49. PANK1

    Plasmid
    #25517
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK1 (PANK1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  50. pJK217

    Plasmid
    #72471
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase with N-terminal His6 tag (H6AaAarA)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72471-plasmid-map-sequence-id-135051

    Enlarge

Showing: 2301 - 2350 of 4184 results