Addgene

We narrowed to 4,301 results for: luciferase

Showing: 2301 - 2350 of 4301 results

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pcDNA3.1+-Tornado-nLuc

Plasmid

#212611

Purpose

For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-nLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEN-luxCDABE

Plasmid

#44918

Depositor

Harry Mobley

Article

Lane et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

sigma 70 promoter

Use

Luciferase

Tags

lux transcriptional fusion

Expression

Mutation

Promoter

em7

Available since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-Tornado-split-nLuc

Plasmid

#212612

Purpose

For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-split-nLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

C373T mutation in CVB3

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxOFP

Plasmid

#229950

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

Depositor

Andy Yeh

Article

Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

Insert

luxOFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxNeon

Plasmid

#229947

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

Insert

luxNeon

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Snail_pGL2

Plasmid

#31694

Depositor

Paul Wade

Article

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Insert

Snail (SNAI1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

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PPRE-pNL1.3

Plasmid

#84394

Purpose

Secreted Luciferase reporter for PPARgamma activity

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

Insert

PPARG response element (PPARG Human)

Use

Tags

secNluc

Expression

Mammalian

Mutation

Promoter

PPRE

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-XNS

Plasmid

#196655

Purpose

Promega's psiCHECK-2 Dual-Luciferase Reporter vector modified to allow the insertion of the target in opposite directions

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Floxed-STOP Fluc

Plasmid

#122962

Purpose

Firefly luciferase (Fluc)-based bioluminescent reporter for Cre-loxP DNA recombination reaction

Depositor

Moritoshi Sato

Article

Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

Insert

pcDNA3.1_Floxed-STOP Fluc

Use

Cre/Lox and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTrex-Luc-Neo

Plasmid

#48336

Purpose

Trasgenically expresses firefly luciferase in T. cruzi with neomycin selection

Depositor

Rick Tarleton

Article

Sanchez-Valdez et al Elife. 2018 Mar 26;7. pii: 34039. doi: 1

Insert

firely luciferase

Use

Trypanasoma cruzi expression

Tags

Expression

Mutation

Promoter

T. cruzi rRNA

Available since

July 20, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pFL-SV40

Plasmid

#115352

Purpose

firefly luciferase (Fluc) reporter gene driven by SV40 promoter

Depositor

Ming-Chih Lai

Article

Lai et al Mol Biol Cell. 2008 Sep;19(9):3847-58. d

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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RSRF-Luc-2wt

Plasmid

#31818

Depositor

Astar Winoto

Article

Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

Insert

MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

MEF2-dependent (-307 to -242 of Nur77/NR4A1)

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
4xM67 pTATA TK-Luc

Plasmid

#8688

Depositor

Jim Darnell

Article

Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

Insert

4 repeated M67 sites

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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luxA+luxB/pRSETB Duet

Plasmid

#197581

Purpose

Expressing luxA and luxB under dual T7 promoter

Depositor

Takeharu Nagai

Article

Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

Insert

LuxA LuxB

Use

Tags

T7 tag and 6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc

Plasmid

#117728

Purpose

Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

NanoLuc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-luxKate

Plasmid

#229951

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

Depositor

Andy Yeh

Article

Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

Insert

luxKate

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCLucf

Plasmid

#37328

Depositor

John Schiller

Article

Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 20

Inserts

Luciferase (luciferase Fly)
GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and SV40

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-luxOFP

Plasmid

#229945

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

Depositor

Andy Yeh

Article

Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

Insert

luxOFP

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMDR1-1202

Plasmid

#37627

Depositor

Kathleen Scotto

Article

Hu et al J Biol Chem. 2000 Jan 28;275(4):2979-85.

Insert

MDR1 promoter (ABCB1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2012

Availability

Academic Institutions and Nonprofits only

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-1748 human cyclin D1 promoter pGL3Basic

Plasmid

#32726

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Reporter gene

Tags

Expression

Mutation

Promoter

-1748CCND1 promoter

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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Slug_pGL2

Plasmid

#31695

Depositor

Paul Wade

Article

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Insert

Slug (SNAI2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pFW11-ffluc

Plasmid

#128487

Purpose

Allelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imaging

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

firefly luciferase gene

Use

Allelic replacement vector

Tags

spy0535 downstream flanking region and spy0535 up…

Expression

Mutation

Promoter

synthetic

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEIAV-SIN6.1 CBLucWm1A

Plasmid

#44163

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Insert

firefly luciferase

Use

Lentiviral and Luciferase

Tags

Expression

Mutation

Promoter

CB (CMV enhancer/chicken beta-actin promoter)

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxGold

Plasmid

#229948

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

Insert

luxGold

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-luxKOk

Plasmid

#229949

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

Insert

luxKOk

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-luxKate

Plasmid

#229946

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

Depositor

Andy Yeh

Article

Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

Insert

luxKate

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD3

Plasmid

#118043

Purpose

GoldenBraid2.0 Universal Part Domesticator #3

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Domestication of dna parts for…

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-LUC (w158-1)

Plasmid

#17473

Purpose

Gateway entry vector, Firefly Luciferase

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-LumiLuc

Plasmid

#126622

Purpose

An engineered luciferase to enhance the bioluminescence of synthetic analog-8pyDTZ

Depositor

Huiwang Ai

Article

Yeh et al ACS Chem Biol. 2019 Apr 17. doi: 10.1021

Insert

LumiLuc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGX-5Dual

Plasmid

#226869

Purpose

transient expression

Depositor

Guoyong Xu

Article

Niu et al Database (Oxford). 2020 Jan 1;2020:baaa0

Insert

Luciferase and hRluc

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pIS1-Vim5UTR-renilla

Plasmid

#38237

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Vim 5'UTR / Renilla (Vim Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Vim 5' UTR fused to renilla ORF

Promoter

Endogenous Vim

Available since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-BRIPO

Plasmid

#215542

Purpose

BRIPO (Bioluminescent Red Indicator for Potassium)

Depositor

Huiwang Ai

Article

Zhao et al J Am Chem Soc. 2024 May 15;146(19):13406

Insert

BRIPO

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2Basic-EcadK1/EpaIMUT/EboxMUT/Ebox2MUT

Plasmid

#19291

Depositor

Eric Fearon

Article

Hajra et al Cancer Res. 2002 Mar 15. 62(6):1613-8.

Insert

E-cadherin

Use

Luciferase

Tags

Expression

Mammalian

Mutation

E-cad promoter sequences from -108 to +125 Ebo…

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pLenti6.2-ccdB-Renilla

Plasmid

#87073

Purpose

Lentiviral Gateway-compatible expression vector backbone with a C-terminal Renilla luciferase

Depositor

Mikko Taipale, Susan Lindquist

Article

Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Renilla luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pNBU2_erm_P1T_DP-GH023 - NanoLuc

Plasmid

#117727

Purpose

Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023 upstream of the NanoLuc reporter

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

NanoLuc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
P1-M-P2

Plasmid

#87091

Purpose

This is the P1-M-P2 portion of the Bcl-2 promoter with a luciferase reporter in pGL3Basic plasmid

Depositor

Yohannes Tesfaigzi

Article

Bredow et al Gene. 2007 Dec 1;404(1-2):110-6. Epub 20

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only
pGWB501NL3F10H

Plasmid

#141286

Purpose

No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, HygR for plant)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Type

Empty backbone

Use

Luciferase; Gateway

Tags

FLAG (x3), HIS (x10), and NLUC

Expression

Plant

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
P526

Plasmid

#139740

Purpose

Individual component of Caffeic Acid biosynthesis pathway, hpaC

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

hpaC

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
phREX1-Luc

Plasmid

#17222

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

Upstream region of human REX1 (ZFP42 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.2-Renilla-ccdB

Plasmid

#87076

Purpose

Lentiviral Gateway-compatible expression vector backbone with an N-terminal Renilla luciferase

Depositor

Mikko Taipale, Susan Lindquist

Article

Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Renilla luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
P525

Plasmid

#139739

Purpose

Individual component of Caffeic Acid biosynthesis pathway, hpaB

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

hpaB

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2-Full

Plasmid

#16012

Depositor

Wafik El-Deiry

Article

Takimoto et al Oncogene. 2000 Mar 30. 19(14):1735-43.

Insert

KILLER/DR5 (TNFRSF10B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-mCherry-GS linker-Zdk1

Plasmid

#125226

Purpose

Expresses mCherry-GS linker-Zdk1 in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

mCherry-GS linker-Zdk1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-BREP

Plasmid

#172337

Purpose

BRET-Based Bioluminescent Red Protein

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BREP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
P527

Plasmid

#139741

Purpose

Individual component of Caffeic Acid biosynthesis pathway, RcTAL

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

TAL

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD2+pG10-90

Plasmid

#170879

Purpose

Phytobrick - pG10-90

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

pG10-90

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFTK052

Plasmid

#171324

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Firefly luciferase reporter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYCeGM0001

Plasmid

#196141

Purpose

Gateway entry vector with Arabidopsis TGA1 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtTGA1_HiBit

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FR_luc

Plasmid

#31098

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

firefly luciferase

Use

Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pGL-Rbl2

Plasmid

#20885

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

Rbl2 (p130) 3'UTR (Rbl2 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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