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pET30a-SARS-CoV2-NP-Nt(47-173)

Plasmid

#165091

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSin-5HT-1.0

Plasmid

#172882

Purpose

make sindbis virus of 5-HT sensor GRAB_5-HT1.0 and express in cells

Depositor

J. Julius Zhu

Article

Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

Insert

5HT SENSOR

Use

Tags

Expression

Mammalian

Mutation

Promoter

sp6

Available since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-ASAP2s

Plasmid

#101275

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAMKIIa

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET32a-Spike-S-4P

Plasmid

#165094

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMIR-CDH1 wtUTR

Plasmid

#25038

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

CDH1 3'UTR (CDH1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pET30a-SARS-CoV2-ORF3b (S23Q)

Plasmid

#165088

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)

Use

Tags

Expression

Bacterial

Mutation

Stop codon to glutamine at 23rd amino acid

Promoter

Available since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMIR-9

Plasmid

#25037

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

miR-9 binding site (MIR9-1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pET32a-Spike-403-505

Plasmid

#165095

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET30a-SARS-CoV2-ORF8

Plasmid

#165089

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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MDH1-PGK-GFP-miR9

Plasmid

#25036

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

mir-9-3 (MIR9-3 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
shNg Lenti FHRC3J1UGW

Plasmid

#92233

Purpose

lentiviral expression of EGFP and Ng shRNA

Depositor

Weifeng Xu

Article

Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

Insert

shRNA targeting Ng

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ292 (AaCas12b)-D570A-TV-MS2-TV

Plasmid

#136381

Purpose

CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b (D570A)-TV-T2A-MCP-TV

Use

CRISPR

Tags

Expression

Plant

Mutation

D570A

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Pcdh15_mini_V8-CD2

Plasmid

#199187

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

mmPcdh15_mini_V8-CD2

Use

AAV

Tags

Expression

Mutation

EC5, EC6, EC8, EC9, EC10 are deleted from msPCDH1…

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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WPXL LEFTY2 gRNA

Plasmid

#101924

Purpose

LEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

Depositor

Danwei Huangfu

Article

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

LEFTY2 enhancer targeting gRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2 Notch1 LNG (CC→SS)-6MT

Plasmid

#41739

Depositor

Raphael Kopan

Article

Mumm et al Mol Cell. 2000 Feb;5(2):197-206.

Insert

Notch-1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS (Notch1 Mouse)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

Contains murine Notch-1 leader peptide (aa 1-23) …

Promoter

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only
WPXL SOX10 gRNA

Plasmid

#101923

Purpose

SOX10 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

Depositor

Danwei Huangfu

Article

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

SOX10 promoter targeting gRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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PBE-NG

Plasmid

#229045

Purpose

Modified nCas9 mediated-base editing with NG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R in Cas9.

Depositor

Huawei Zhang

Article

Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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B2M-SDMutation-EhT

Plasmid

#215546

Purpose

Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)

Use

CRISPR

Tags

Expression

Mutation

The second base of the first intron of B2M (From …

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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B2M-SDMutation-EPG

Plasmid

#215545

Purpose

Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)

Use

CRISPR

Tags

Expression

Mutation

The second base of the first intron of B2M (From …

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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B2M-SDMutation-gRNA

Plasmid

#215547

Purpose

gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

B2M (B2M Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge