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pET21-pelB-LaTdT-9

Plasmid

#228977

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-9

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_tKiMBImut-T2A-caMEK

Plasmid

#199579

Purpose

Express tKiMBImut(AA) and caMEK in an AAV vector

Depositor

Michael Lin

Article

Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

Inserts

ERK tdTomato-Kinase-Modulated Bioluminescent Indicator (mutant)
constitutively active MEK

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD

Plasmid

#199447

Purpose

Expession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signal

Depositor

Baohui Chen

Article

Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

Insert

dSaCas9-10xGCN4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human ubiquitin C (UBC)

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-GFP-VP16

Plasmid

#183935

Purpose

PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-GFP-VP16 (UL48 Synthetic)

Use

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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MYH11-gRNA1

Plasmid

#132587

Purpose

gRNA used for knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele (MYH11-NanoLuc-2A-tdTomato vector) )

Depositor

James Thomson

Article

Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

Insert

MYH11-gRNA1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLX304-IRES-Td

Plasmid

#176296

Purpose

pLX304 backbone with an IRES-Tdtomato cassette subcloned into the NheI-AgeI sites

Depositor

William Kaelin

Article

Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

Type

Empty backbone

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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Fireworks RFP[PTC+] (AVA2626)

Plasmid

#85444

Purpose

Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.

Depositor

Joan Steitz

Article

Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

Insert

tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR

Use

Minimal backbone fragment for low-copy bacterial …

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pFIN-XOPS-tdTOM-MOPS-GFP

Plasmid

#44347

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Inserts

XOPS-tdTOM
MOPS-GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Xenopus Opsin (XOPS) (pos 3131-4495) and mouse op…

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pMADM-alpha

Plasmid

#36890

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdTomato-3Myc
beta-globin intron
Neo
ATG (T)
GFP

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, deleted…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-dTom-nlsdTom

Plasmid

#135630

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MIP 227 CoMiP 4in1 wo shRNA p53

Plasmid

#63727

Purpose

To express Oct4, Klf4, Sox2, and c-Myc. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

Depositor

Joseph Wu, Mark Kay

Article

Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

Insert

Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC Human)

Use

Tags

IRES-dtomato

Expression

Mammalian

Mutation

codon-optimised

Promoter

SFFV

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E6-dTom-nlsdTom

Plasmid

#135641

Purpose

AAV vector to drive the expression of dTomato in VIP cortical interneurons under the control of the E6 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV1

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E5-dTom-nlsdTom

Plasmid

#135640

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E5 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-S5E8-dTom-nlsdTom

Plasmid

#135643

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E8 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-S5E1-dTom-nlsdTom

Plasmid

#135637

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E1 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-S5E3-dTom-nlsdTom

Plasmid

#135638

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E3 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-S5E4-dTom-nlsdTom

Plasmid

#135639

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E4 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-S5E7-dTom-nls-dTom

Plasmid

#135642

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E7 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-S5E9-dTom-nls-dTom

Plasmid

#135644

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E9 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-S5E10-dTom-nlsdTom

Plasmid

#135645

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E10 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge