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Rare driver mutations in head and neck squamous cell carcinomas converge on NOTCH signaling.
Loganathan SK, Schleicher K, Malik A, Quevedo R, Langille E, Teng K, Oh RH, Rathod B, Tsai R, Samavarchi-Tehrani P, Pugh TJ, Gingras AC, Schramek D
Science. 2020 Mar 13;367(6483):1264-1269. doi: 10.1126/science.aax0902.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
158030pLKO-Cre stuffer v3lenti-viral construct with Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9
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158032pLKO-Cre stuffer v4lenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9
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158033pCRISPR-Cre stuffer v4lenti-viral construct with Cas9, Cre recombinase and U6 driven sgRNA casette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)
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158034pLKO-Cre sgAdam10 v3lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Adam10
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158035pLKO-Cre sgRipk4 v3lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Ripk4
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158036pLKO-Cre sgUroD v4lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse UroD
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158038pLKO-Cre sgItgb5 v3lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Itgb5
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158046pLKO-CreERT2 stuffer v3lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9
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158047pLKO-CreERT2 sgAdam10 v3lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Adam10. NO Cas9
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158048pLKO-CreERT2 sgRipk4 v3lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Ripk4. NO Cas9
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158049PLX306 iCre tetO NICD rtTAlenti-viral construct with Cre recombinase and doxycycline inducible expression of mouse Notch intracellular domain
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158050PLX306 iCre tetO tomato rtTAlenti-viral construct with Cre recombinase and doxycycline inducible expression of tdTomato
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158602pLKO-Cre sgAmotl2 v3lenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2
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