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pL-CRISPR.EFS.GFP

Plasmid

#57818

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

EFS and hU6

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.GFP

Plasmid

#57827

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pMax_GFP

Plasmid

#177825

Purpose

Host Cell Reactivation (HCR) control GFP plasmid (pMax backbone)

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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ERmoxGFP

Plasmid

#68072

Purpose

mammalian expression of ER localized moxGFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Insert

moxGFP

Use

Tags

KDEL ER retention sequence and bovine prolactin s…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pimEJ5GFP

Plasmid

#44026

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

Insert

EJ5GFP egfp-based chromosomal break reporter

Use

Tags

Expression

Mammalian

Mutation

pCAGGS promoter and eGFP separated by pgkPURO cas…

Promoter

pCAGGS

Available since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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4xnrUAS:GFP

Plasmid

#61373

Purpose

4xnrUAS driving GFP

Depositor

Marnie Halpern

Article

Akitake et al Dev Biol. 2011 Apr 15;352(2):191-201. do

Insert

4xnr UAS:GFP

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pInducer20_EGFP

Plasmid

#228878

Purpose

Tet-inducible lentiviral vector for expression of optimized GFP

Depositor

Ramiro Iglesias-Bartolome

Article

Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP

Plasmid

#174893

Purpose

amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Insert

mCherry and EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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SFG.wtCNa_opt.IRES.eGFP

Plasmid

#22489

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Sep 21. ():.

Insert

codon optimised wild type Calcineurin A (PPP3CA Human)

Use

Retroviral

Tags

Expression

Mutation

Codon Optimised wtCNa

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.Luciferase.IRES.GFP

Plasmid

#169308

Purpose

Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Luciferase

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mutation

Promoter

SFFV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP

Plasmid

#176015

Purpose

Mammalian expression of EGFP. Parton lab clone DFS

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-AR Q0

Plasmid

#86427

Purpose

Fluorescent human androgen receptor (fused to EGFP) with deleted polyglutamine region

Depositor

Michael Mancini

Article

Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

Insert

androgen receptor (AR) (AR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the poly-glutamine expansion

Promoter

CMV

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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hprtSAGFP

Plasmid

#41594

Purpose

In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences

Depositor

Maria Jasin

Article

Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.

Insert

SA-GFP reporter

Use

Mouse Targeting; Dsb repair reporter substrate

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pINDUCER20_mGFP

Plasmid

#190027

Purpose

Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal mEGFP

Depositor

Andrew Tutt

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP

Plasmid

#165830

Purpose

EGFP green fluorescent protein CDS

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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LacZ_pHIVEGFP

Plasmid

#191251

Purpose

Expresses LacZ in mammalian cells (used as a control)

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

LacZ

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pTD103luxI_sfGFP

Plasmid

#48885

Purpose

Positive feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

LuxI, sfGFP, and LuxR

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Mutation

Promoter

pLux

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pB_tetO7_sfGFP

Plasmid

#196616

Purpose

Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

Superfolder GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

tetO7 promoter

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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EF.PGK.GFP

Plasmid

#17618

Depositor

Linzhao Cheng

Article

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2_ubi:EGFP

Plasmid

#27323

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

ubiquitin:EGFP reporter

Use

Tol2 transposon

Tags

Expression

Mutation

Promoter

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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