We narrowed to 11,368 results for: VARS

1. pGL0_20 [panARS-opt ORI]

   Plasmid

   #198933

   Purpose

   Level 0 part

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   panARS-opt ORI
   

   Use

   Synthetic Biology

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-ND1 Left -GSVG-UGI

   Plasmid

   #187412

   Purpose

   human mitochondrial DNA ND1 target TALE

   Depositor

   Jin-Soo Kim

   Article

   Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

   Insert

   DddA GSVG variant
   

   Expression

   Mammalian

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHis17_ParM _1

   Plasmid

   #78201

   Purpose

   Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

   Depositor

   Martin Webb

   Article

   Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

   Insert

   parM (K33A I27C T174A T175N C287A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   K33A I27C T174A T175N C287A

   Promoter

   unknown

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDEST14-DoubleCatcher-H-Lock

   Plasmid

   #216285

   Purpose

   Expresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher H-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CMV-CitronRH

   Plasmid

   #158545

   Purpose

   Mammalian expression of nonbinding variant of direct-response citrate sensor

   Depositor

   Robert Campbell

   Article

   Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

   Insert

   CitronRH
   

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. eNme2C-CBE6c

   Plasmid

   #215831

   Purpose

   Express CBE6c (with eNme2-C variant) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6c-eNme2C-2xUGI
   

   Expression

   Mammalian

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJH462

   Plasmid

   #172154

   Purpose

   pylBCD variant in pBAD backbone

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Para (Sumo-tagged pylBCD); AraC
   

   Expression

   Bacterial

   Mutation

   SUMO tag fused to N-terminus of PylB

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJH643

   Plasmid

   #172168

   Purpose

   Reporter plasmid for pylBCD variant in pBAD backbone

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Para sfGFP.3TAG; Plpp pylS; PproK pylT; AraC; Cam
   

   Expression

   Bacterial

   Mutation

   sfGFP(N39*, N135*, Y151*)

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p458 VQR

   Plasmid

   #101727

   Purpose

   Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Expression

   Mammalian

   Mutation

   VQR

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mFGF8b

    Plasmid

    #22090

    Depositor

    Gail Martin

    Article

    Crossley et al Development. 1995 Feb . 121(2):439-51.

    Insert

    FGF8 (Fgf8 Mouse)
    

    Use

    Bacterial

    Available Since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pGL2_163

    Plasmid

    #199095

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pAMβ1 ORI plus NGS barcode 3, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-6xHis-GFP(+12)

    Plasmid

    #199161

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+12)
    

    Tags

    MGHHHHHHGGA

    Mutation

    E16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-6xHis-GFP(+18)

    Plasmid

    #199162

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+18)
    

    Tags

    MGHHHHHHGGA

    Mutation

    E16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ii-Str_neomycin

    Plasmid

    #65308

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Invariant chain (p33)
    

    Expression

    Mammalian

    Mutation

    Streptavidin in its lumenal part

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSX2720

    Plasmid

    #200764

    Purpose

    Expresses Superfolder GFP-tag fused single change variable fragment antibody of GCN4 (Suntag) with the tissue-specific promoter col-19 in C. elegans.

    Depositor

    Suhong Xu

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    scFv-sfGFP
    

    Expression

    Worm

    Promoter

    col-19

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LT3GEN

    Plasmid

    #111173

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    T3G

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CMV-CitroffRH

    Plasmid

    #158546

    Purpose

    Mammalian expression of nonbinding variant of inverse-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    CitroffRH
    

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Ii-Str_puromycin

    Plasmid

    #65309

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Invariant chain (p33)
    

    Expression

    Mammalian

    Mutation

    Streptavidin in its lumenal part

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL2_166

    Plasmid

    #199098

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    RK2 ORI plus NGS barcode 12, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-LRF

    Plasmid

    #66936

    Purpose

    Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

    Depositor

    Alvaro Monteiro

    Article

    Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV-40

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only