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gnu-GFP 9A

Plasmid

#113006

Purpose

P element vector for transposon insertion of gnu-GFP 9A into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

P insertion vector

Tags

GFP

Mutation

9 CycB/CDK1 sites mutated to A

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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gnu-GFP WT

Plasmid

#113005

Purpose

P element vector for transposon insertion of gnu-GFP WT into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

P insertion vector

Tags

GFP

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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6xmyc_cort_UAS-P

Plasmid

#112991

Purpose

P element vector for transposon insertion of Myc-tagged Drosophila cort (Cdc20) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Pesin et al PLoS Genet. 2007 Nov;3(11):e202. doi: 10

Insert

cort (Cdc20) (cort Fly)

Use

P insertion vector

Tags

6X myc epitope

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1 gnu 9A

Plasmid

#113000

Purpose

For protein expression and purification of full-length Drosophila gnu 9A

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Expression

Bacterial

Mutation

9 CycB/CDK1 sites mutated to A

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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full length png in pGEX4t

Plasmid

#112974

Purpose

For protein expression and purification of full-length Drosophila png

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

png (png Fly)

Tags

His

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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gnu cDNA LD12084

Plasmid

#112973

Purpose

Cloning vector carrying the gnu cDNA

Depositor

Terry Orr-Weaver

Article

Lee et al Genes Dev. 2003 Dec 1;17(23):2979-91. do

Insert

gnu (gnu Fly)

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R7R8

Plasmid

#111041

Purpose

Plasmid expresses the talin2 rod domains R7 and R8

Depositor

Ben Goult

Article

Bouchet et al Elife. 2016 Jul 13;5. doi: 10.7554/eLife

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R7

Plasmid

#111039

Purpose

Plasmid expresses the talin2 rod domain R7 a 5 helix bundle.

Depositor

Ben Goult

Article

Bouchet et al Elife. 2016 Jul 13;5. doi: 10.7554/eLife

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCML 221

Plasmid

#98587

Purpose

pMS2-CYFP. Expresses MS2 protein-CYFP fusion in E. coli for TriFC assay (kanR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Insert

MS2

Promoter

pLacO

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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nucHCB1

Plasmid

#97424

Purpose

Nuclear Huntingtin Chromobody 1: "Happ1" intrabody recognizing amino acids 41–81 of huntingtin, fused to eYFP and nuclear localization signal.

Depositor

Ray Truant

Article

Maiuri et al Hum Mol Genet. 2016 Dec 25. pii: ddw395.

Insert

nucHCB1

Tags

NLS and YFP

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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