We narrowed to 26,138 results for: Vit

1. MYO3B gRNA (BRDN0001145445)

   Plasmid

   #75569

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MYO3B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MYO3B (MYO3B Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pScalps_puro_mIL-2Rα ST

   Plasmid

   #59919

   Purpose

   Expresses mouse interleukin-2 receptor alpha chain mutated

   Depositor

   Silvia Monticelli

   Article

   Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

   Insert

   interleukin 2 receptor alpha (Il2ra Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   changed sequence of the intracellular (C-terminal…

   Promoter

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

3. AB.pCCL.sin.cPPT.U6.miR-874-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46637

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-874 Decoy (MIR874, Mir874 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

4. AB.pCCL.sin.cPPT.U6.miR-330-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46611

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-330 Decoy (MIR330, Mir330 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

5. AB.pCCL.sin.cPPT.U6.miR-744-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46633

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-744 Decoy (MIR744, Mir744 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

6. AB.pCCL.sin.cPPT.U6.miR-195-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46598

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-195 Decoy (MIR195, Mir195a Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

7. HsCLYBL(aa23-340) D320N-His

   Plasmid

   #111289

   Purpose

   bacterial expression

   Depositor

   Vamsi Mootha

   Article

   Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

   Insert

   human CLYBL ( aa23-340) D320N codon optimized for bacterial expression (CLYBL Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBacHT (His10-4xFRB-SNAP-Lck G2A)

   Plasmid

   #177887

   Purpose

   Baculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2A

   Depositor

   Ron Vale

   Article

   Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

   Insert

   Lck (LCK Human)
   

   Use

   Tags

   10xHis-TEV-4xFRB-SNAP tag

   Expression

   Insect

   Mutation

   G2A

   Promoter

   Polyhedrin

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-N-HA-NEK7

   Plasmid

   #75142

   Purpose

   Expresses N-terminal HA-tagged NEK7 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. TRIM28 gRNA (BRDN0001162487)

    Plasmid

    #76139

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM28

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRIM28 gRNA (BRDN0001146162)

    Plasmid

    #76142

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM28

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-hSyn-GRAB-rDA3h

    Plasmid

    #208703

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3h
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti6.3/TO/CD9-GFP

    Plasmid

    #104402

    Purpose

    Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal

    Depositor

    Jens Gruber

    Article

    Boker et al Mol Ther. 2018 Feb 7;26(2):634-647. doi:

    Insert

    CD9 (CD9 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRibo-Sec-APEX2m

    Plasmid

    #176844

    Purpose

    Expression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)

    Depositor

    Jessica Seeliger

    Article

    Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

    Insert

    pRibo-Sec-APEX2m
    

    Use

    Inducible

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRibo, theophilline riboswitch inducible hsp60 pr…

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-GST-BcPC-PLC

    Plasmid

    #224255

    Purpose

    Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli

    Depositor

    Chiaki Murakami

    Article

    Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:

    Insert

    Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
    

    Use

    Tags

    GST and HRV-3C (LEVLFQGP) site

    Expression

    Bacterial

    Mutation

    The gene of Bacillus cereus ATCC 14579 was used f…

    Promoter

    lac promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-Dio-ASAP5-Kv2.1-WPRE

    Plasmid

    #225708

    Purpose

    Cre dependent soma-targeted expression of the genetically encoded voltage indicator ASAP5

    Depositor

    Michael Lin

    Article

    Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

    Insert

    ASAP5-Kv2.1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1α

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-TRE-DIO-hM4Di-mCherry

    Plasmid

    #182532

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The hM4Di receptor expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Yu et al Science. 2022 Jul;377(6601):63-72. doi:

    Insert

    AAV transgene - hSyn promoter-tet-flex-hM4Di-cherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    hM4Di-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    tetO promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. T7_CC_PE7_IVT_Template

    Plasmid

    #223022

    Purpose

    Template for in vitro transcription of PE7. For HSPC-related experiments.

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. GAL1-DuET

    Plasmid

    #213248

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GAL1 (GALR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-AKAP79-AKAR4

    Plasmid

    #181957

    Purpose

    Genetically encoded FRET-based PKA activity reporter fused to AKAP79.

    Depositor

    Jin Zhang

    Article

    Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

    Insert

    AKAP79-AKAR4 (AKAP5 Human)
    

    Use

    Tags

    AKAP79, Cerulean, HIS tag, and cpVenus(E172)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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