We narrowed to 10,053 results for: ADA

1. RS02060_pMQ30_KO_Kan

   Plasmid

   #185391

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS02060 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS02060 upstream flanking homology region
   RR42_RS02060 downstream flanking homology region
   

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. RS28935_pMQ30_KO_Kan

   Plasmid

   #185392

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS28935 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS28935 upstream flanking homology region
   RR42_RS28935 downstream flanking homology region
   

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. RS18965_pMQ30_KO_Kan

   Plasmid

   #185394

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS18965 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS18965 upstream flanking homology region
   RR42_RS18965 downstream flanking homology region
   

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. RS18970_pMQ30_KO_Kan

   Plasmid

   #185395

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS18970 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS18970 upstream flanking homology region
   RR42_RS18970 downstream flanking homology region
   

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW-MD-mTurquoise

   Plasmid

   #193094

   Purpose

   constitutive expression of the mitosis degradation (MD) domain of cyclin B1 fused to mTurquoise in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

   Insert

   MD-mTurquoise
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Ubc

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pW228-lenti-sg1-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #189944

   Purpose

   Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg1-Serpinh1) with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

   Insert

   NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #1
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pW229-lenti-sg2-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #189945

   Purpose

   Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg2-Serpinh1) with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

   Insert

   NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pW230-lenti-sg3-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #189946

   Purpose

   Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg3-Serpinh1) with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

   Insert

   NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pW317-lenti-sg1-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #189947

   Purpose

   Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg1-Itga9) with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

   Insert

   NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #1
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pW318-lenti-sg2-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #189948

    Purpose

    Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg2-Itga9) with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

    Insert

    NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pW319-lenti-sg3-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #189949

    Purpose

    Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg3-Itga9) with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

    Insert

    NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pW401-lenti-sg1-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #189950

    Purpose

    Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg1-Itgb1) with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

    Insert

    NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pW402-lenti-sg2-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #189951

    Purpose

    Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg2-Itgb1) with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

    Insert

    NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pW403-lenti-sg3-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #189952

    Purpose

    Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg3-Itgb1) with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

    Insert

    NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. QUAS-0-T7-TetO-GFP_T7-LacO-TetR_ColE1

    Plasmid

    #171663

    Purpose

    QUAS directly upstream of a T7 promoter with a TetO operator. Expression of TetR in BL21(DE3) E. coli. GFP is expressed according to presence of aTc. Contains the ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-T7-TetO-GFP ssrA-T7 Stop- T7-LacO-TetR-T7 Stop
    

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pILGFP8EFA

    Plasmid

    #185854

    Purpose

    Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
    

    Expression

    Yeast

    Mutation

    DHFR P66L

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. C335

    Plasmid

    #141125

    Purpose

    Expresses chimeric repressor (RbsR-L) under Cin promoter

    Depositor

    Matthew Bennett

    Article

    Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

    Inserts

    LLhR
    AiiA (AQ980_RS12585 Synthetic)
    

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Chimera: DNA binding domain of LacI and ligand bi…

    Promoter

    engineered cin promoter (responds to C14-HSL) and…

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-FNLSHF1-P2A-Puro

    Plasmid

    #136901

    Purpose

    Lentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHF1
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Mutation

    R661A,Q695A,Q926A

    Promoter

    EF1s

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBEAST_J23101-vdh

    Plasmid

    #128134

    Purpose

    The cell-free adapted backbone, pBEAST, expressing vdh (the enzyme converting benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    vdh
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23101

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBEAST_J23101-bphD

    Plasmid

    #128138

    Purpose

    The cell-free adapted backbone, pBEAST, expressing bphD (the enzyme converting 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    bphD
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23101

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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