We narrowed to 27,150 results for: cat

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pcDNA4 myc PGC-1 alpha

Plasmid

#10974

Purpose

Mammalian expression of PGC-1a with myc and his tags

Depositor

Toren Finkel

Article

Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

Insert

PGC-1 alpha (PPARGC1A Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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SpdCas9 KRAB

Plasmid

#188499

Purpose

Expresses FLAG tagged SpdCas9 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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L_NLS 41kDa

Plasmid

#201342

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A4-EGFP-2PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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Diffusive 67kDa

Plasmid

#201338

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

EGFP-6PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMax_mOrange

Plasmid

#177827

Purpose

Host Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE BRG1

Plasmid

#1959

Depositor

Robert Kingston

Article

Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

Insert

BRG1 (SMARCA4 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

E1262K R1575Q

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only

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SadCas9 VPR

Plasmid

#188514

Purpose

Expresses FLAG tagged SadCas9 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/N580A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_MMP-9-HA

Plasmid

#121172

Purpose

Expression of mouse MMP9

Depositor

M. Elizabeth Fini, Shinwu Jeong

Article

Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

Insert

MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)

Tags

HA

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV immediate/early

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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LacZ in pLX304

Plasmid

#42560

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

LacZ

Use

Lentiviral

Tags

2xV5

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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pPolI_SARS-CoV-2_NLuc

Plasmid

#183953

Purpose

Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

Depositor

AJ te Velthuis

Article

Vial et al bioRxiv 2021.06.28.450211

Insert

SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)

Expression

Mammalian

Promoter

Mammalian Pol I

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-ATF6

Plasmid

#11975

Purpose

For mammalian expression of human ATF6 with a 3x FLAG tag

Depositor

Ron Prywes

Article

Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

Insert

ATF6 (ATF6 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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pEvolvR-enCas9-PolI3M-TBD

Plasmid

#113077

Purpose

Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber, David Schaffer

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI3M-TBD

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-dCasRx-METTL3-PURO

Plasmid

#174218

Purpose

Expresses dCasRx-METTL3 in mammalian cell

Depositor

Qi Xie

Article

Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

Insert

dCasRx-METTL3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

pMSCV

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMax_mPlum

Plasmid

#177830

Purpose

Host Cell Reactivation (HCR) control mPlum plasmid (pMax backbone)

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mPlum

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pHRSin_hTCRαγεζ

Plasmid

#187351

Purpose

Lentiviral expression of GPa3b17 hTCR alpha, and truncated hCD3 (gamma, epsilon, and zeta)

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR alpha, and truncated hCD3 (gamma, epsilon, and zeta)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-UTX-F

Plasmid

#17438

Purpose

Expression of UTX in mammalian cells

Depositor

Kai Ge

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

Insert

UTX (KDM6A Human)

Tags

6xMyc and Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

Plasmid

#199349

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP-SUMOEu

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CAG-FLEX-BFP2-(mCherry)’

Plasmid

#115234

Purpose

Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

mTagBFP2 and mCherry

Expression

Mammalian

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDest-Sumo

Plasmid

#106980

Purpose

LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag

Depositor

Han Choe

Article

Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10

Type

Empty backbone

Tags

His8-Sumo

Expression

Bacterial

Promoter

T7

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.tgoGAP1

Plasmid

#78737

Purpose

Expression of Ca2+ sensor GAP1 in the trans Golgi apparatus

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

tgoGAP1

Tags

N terminal 81 amino acids of human galactosyltran…

Expression

Mammalian

Mutation

D117A/D119A/D163A in aequorin moiety

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only