Addgene

We narrowed to 3,661 results for: atr;

Showing: 1411 - 1440 of 3661 results

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L1_NOP1:mS-N7-Ck2

Plasmid

#136126

Purpose

L1 in position 2, example of weak promoter

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpNOP1:mScarletI-N7_3tNos-35S

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1_UBE2:mT-N7-Ck2

Plasmid

#136127

Purpose

L1 in position 2, control of expression

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mTurquoise2-N7_3tNos-35S

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1_UBE2:mT-N7-Ck3

Plasmid

#136128

Purpose

L1 in position 3, control of expression

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mTurquoise2-N7_3tNos-35S

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1_UBE2:eG-Lt-Ck3

Plasmid

#136129

Purpose

L1 in position 3, mark cell outline with eGFP

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:eGFP-Lti6b_3tNos-35S

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CTAG_eGFP-HDEL

Plasmid

#136097

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_eGFP(noATG,noStop)-HDEL

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CTAG_linker-Lti6b

Plasmid

#136099

Purpose

Plasma Membrane targeting protein for C-term fusion with CDS12 without linker.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSerGly10xAla_Lti6b

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_linker-mVenus

Plasmid

#136101

Purpose

FP for C-term fusion with CDS12 without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSerGly10xAlaGlySer_mVenus

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_linker-N7

Plasmid

#136102

Purpose

Nuclear targeting peptide for C-term fusion with CDS12 without linker.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSer4xAla_N7

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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5UTR_MpNOP1

Plasmid

#136103

Purpose

5UTR for cloning with PROM_MpNOP1

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

5UTR_MpNOP1 Mapoly0008s0036

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_mTurquoise2-HDEL

Plasmid

#136105

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_mTurquoise2(noATG,noStop)-HDEL

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_mVenus-HDEL

Plasmid

#136107

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_mVenus(noATG,noStop)-HDEL

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_NLS-SV40

Plasmid

#136108

Purpose

CTAG Nuclear localiation signal SV40 for fusion with CDS12

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_NLS-SV40

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsB_spacer

Plasmid

#136076

Purpose

Use when making odd level constructs (L3,...) if pCsB position (position 2) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS12_mTurquoise2-linker

Plasmid

#136090

Purpose

FP for N-term fusion with a CTAG without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mTurquoise2(noStop)-SerGly10xAlaSer

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS12_mVenus-linker

Plasmid

#136092

Purpose

FP for N-term fusion with a CTAG without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mVenus(noStop)-SerGly10xAlaSer

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS12_TP-MpSIG2

Plasmid

#136093

Purpose

Chloroplast transit peptide from MpSIG2. For N-term fusion with a CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

TP-MpSIG2, Mapoly0214s0004

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsA_spacer

Plasmid

#136075

Purpose

Use when making odd level constructs (L3,...) if pCsA position (position 1) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pRS426-CeLACE-1

Plasmid

#174523

Purpose

Express C.elegans LACE-1 in yeast cells

Depositor

Oleh Khalimonchuk

Article

Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

Insert

lace-1 (C30F12.2 Nematode)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

MET25

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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CMYA5/pBSSK+

Plasmid

#97205

Purpose

CMYA5 (NM_153610, nt 12205 to 12279) in pBSSK+

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

July 28, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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CMYA5/pENTR3C

Plasmid

#97408

Purpose

CMYA5 (NM_153610, nt 12205 to 12279) in pENTR3C, Gateway Entry vector

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

July 18, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pRS425-M25-Afg1

Plasmid

#61720

Purpose

yeast Afg1 AAA protein in yeast expression vector

Depositor

Oleh Khalimonchuk

Article

Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

Insert

Afg1 (AFG1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only
KHBD00582

Plasmid

#39666

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5369 (CG5369 Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
pCMV(CAT)T7-SB100

Plasmid

#34879

Depositor

Zsuzsanna Izsvak

Article

Mates et al Nat Genet. 2009 Jun;41(6):753-61. Epub 2

Insert

SB100X transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pBA-mCherry-EGFP-PIM

Plasmid

#111758

Purpose

Low level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition

Depositor

Lukas Kapitein

Article

Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:

Insert

2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP

Use

Tags

Expression

Mammalian

Mutation

Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

Promoter

chicken beta-actin

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGW1-mCherry-EGFP-PIM

Plasmid

#111759

Purpose

High expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition

Depositor

Lukas Kapitein

Article

Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:

Insert

2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP

Use

Tags

Expression

Mammalian

Mutation

Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

Promoter

CMV

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ManII-SBP-mCherry

Plasmid

#65259

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-EGFP-KASH

Plasmid

#154373

Purpose

Vector for Cre-dependent expression of EGFP tagged KASH protein domain

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

EGFP-KASH

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-mScarlet

Plasmid

#207960

Purpose

Vector for overexpression of the mScarlet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEBG‐AMPK α1(1‐312)

Plasmid

#27632

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

AMPK α1(1‐312) (Prkaa1 Rat)

Use

Tags

GST

Expression

Mammalian

Mutation

Constitutively  active  by  truncation  after  am…

Promoter

Available since

May 18, 2011

Availability

Academic Institutions and Nonprofits only

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CROPseq-tRFP

Plasmid

#230938

Purpose

Modified version of the original CROP-seq vector (Addgene #86708), expressing tRFP instead of puroR for FACS

Depositor

Hyejung Won

Article

Lee et al Cell. 2025 Jan 22:S0092-8674(24)01435-1.

Type

Empty backbone

Use

Lentiviral

Tags

TagRFP

Expression

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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