We narrowed to 9,400 results for: Coli

1. pKDsgRNA-pfkB

   Plasmid

   #102284

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   pfkB gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICSL80037

   Plasmid

   #68260

   Purpose

   Level 0 Golden Gate Part: Coding sequence, neomycin phophotransferase II (Escherichia coli)

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Coding sequence, neomycin phophotransferase II (Escherichia coli)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYihI-N-term K-R

   Plasmid

   #233091

   Purpose

   Expression of GST-YihI with N-term lysines (K) mutated to arginine (R)

   Depositor

   Michael Downey

   Article

   Baijal et al bioRxiv 2025.02.12.637445

   Insert

   YihI with N-term lysines (K) mutated to arginine (R)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   N-terminal lysine residues mutated to arginine

   Promoter

   Available Since

   May 12, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pYihI-All K-R

   Plasmid

   #233094

   Purpose

   Expression of GST-YihI with all lysines (K) mutated to arginine (R)

   Depositor

   Michael Downey

   Article

   Baijal et al bioRxiv 2025.02.12.637445

   Insert

   GST-YihI with all lysines (K) mutated to arginine (R)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   GST-YihI with all lysines (K) mutated to arginine…

   Promoter

   Available Since

   May 9, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pRAR092-L31

   Plasmid

   #185045

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L31 coding region, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRAR112

   Plasmid

   #185048

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region K2A mutation, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31p
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K2A amino acid mutation

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRAR113

   Plasmid

   #185049

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region H6A mutation, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31p
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H6A amino acid mutaitons

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRAR114

   Plasmid

   #185050

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region E8A mutation, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31p
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E8A amino acid mutation

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRAR089-L36p

   Plasmid

   #185042

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L36p coding region, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L36p
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRAR090-L36

    Plasmid

    #185043

    Purpose

    J23108(spel), pHP14 stability hairpin, strong RBS, L36 coding region, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L36
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRAR091-L31p

    Plasmid

    #185044

    Purpose

    J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31p
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr

    Plasmid

    #218767

    Purpose

    encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG

    Depositor

    Abhishek Chatterjee

    Article

    Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

    Insert

    EcTyrRS-pAAFRS-9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y37G, L71V, D182C, F183Y, L186C, D265R

    Promoter

    tacI

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMCSG7-10xHis-ecRNHI*

    Plasmid

    #219523

    Purpose

    bacterial expression of cysteine-free variant of the E. coli ribonuclease HI gene

    Depositor

    Arthur Palmer

    Article

    Bhattacharya et al J Biomol NMR. 2024 Jun 10. doi: 10.1007/

    Insert

    cysteine-free variant of the E. coli ribonuclease HI gene
    

    Use

    Tags

    N terminal 10xHis Tag

    Expression

    Bacterial

    Mutation

    Cysteine free variant

    Promoter

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBN095

    Plasmid

    #173765

    Purpose

    express E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    delete motif 3

    Promoter

    araBAD

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBN215

    Plasmid

    #167962

    Purpose

    express E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Y715E, Y717E, Y718E

    Promoter

    araBAD

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBN216

    Plasmid

    #167963

    Purpose

    express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Y713E, Y715E, Y717E, Y718E

    Promoter

    araBAD

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBN217

    Plasmid

    #167964

    Purpose

    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Y708E, Y713E, Y715E, Y717E, Y718E

    Promoter

    araBAD

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBN261

    Plasmid

    #167966

    Purpose

    express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Y713E, Y715E, Y717E

    Promoter

    araBAD

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBN262

    Plasmid

    #167967

    Purpose

    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Y708E, Y713E, Y715E, Y717E

    Promoter

    araBAD

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBRC901

    Plasmid

    #167948

    Purpose

    express wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge