We narrowed to 1,842 results for: rigi

1. pRAM24

   Plasmid

   #234313

   Purpose

   pBBR1 origin cat-RNA U1376

   Depositor

   James Chappell

   Article

   Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

   Insert

   U1367 Targeting RAM, Barcode 1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P. CymRC

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAX2

   Plasmid

   #117398

   Purpose

   allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch

   Depositor

   Karen Guillemin

   Article

   Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpCas9-CBE6c-IVT

   Plasmid

   #215835

   Purpose

   Template for in vitro transcription of CBE6c (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6c-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. ABE-RA2.0

   Plasmid

   #208299

   Purpose

   Expresses ABE-RA2.0 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA2.0-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…

   Promoter

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ABE-RA3.1

   Plasmid

   #208302

   Purpose

   Expresses ABE-RA3.1 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA3.1-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E27D, R47K, P48A, R51H, I76F, D108G, K110R, H122R…

   Promoter

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAC-BA-lacZ

   Plasmid

   #13423

   Depositor

   Keith Joung

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Insert

   BA
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAM5411

   Plasmid

   #132663

   Purpose

   RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance

   Depositor

   Susan Golden

   Article

   Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

   Inserts

   aadA
   YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PaadA and PconII

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. TALED_Left-stuffer-1397C-8e

   Plasmid

   #187169

   Purpose

   original vector to make all constructs

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

   Insert

   Left-stuffer-1397C-8e
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TALED_Right-stuffer-1397N-UGI

   Plasmid

   #187173

   Purpose

   original vector to make all constructs

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

   Insert

   Right-stuffer-1397N-UGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. eNme2C-CBE6b

    Plasmid

    #215830

    Purpose

    Express CBE6b (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6b-eNme2C-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pY3

    Plasmid

    #50606

    Purpose

    Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5

    Depositor

    Jay Keasling

    Article

    Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

    Inserts

    tyrB
    tyrA
    aroC
    aroA
    aroL
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Nov. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJUMP23-1A(sfGFP)

    Plasmid

    #126970

    Purpose

    Level 1 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pgRNA

    Plasmid

    #215560

    Purpose

    Constitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanR

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    gRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSB1C3-Lux

    Plasmid

    #109383

    Purpose

    Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pARABAD-LUX operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-Universal

    Plasmid

    #52694

    Purpose

    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

    Depositor

    Sebastian Lourido

    Inserts

    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    

    Use

    CRISPR; Toxoplasma gondii

    Tags

    3X FLAG and Nuclear localization signal

    Expression

    Mutation

    Promoter

    TgTUB1 and Toxoplasma U6

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMR-A-5

    Plasmid

    #138519

    Purpose

    P3c

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMR-A-3

    Plasmid

    #138517

    Purpose

    P3a

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pALS3-Ma-sfGFP-TAG150

    Plasmid

    #212121

    Purpose

    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    sfGFP-150TAG
    M. alvus Pyl-tRNA(6)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    araC and lpp

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBT138b-proB

    Plasmid

    #138515

    Purpose

    P3f

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJUMP27ts-1A(sfGFP)

    Plasmid

    #126975

    Purpose

    Level 1 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    RepA A56V thermosensitive mutation

    Promoter

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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