We narrowed to 12,028 results for: LEA

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pT7-EGFP-C1-HsNot1iso1_1595-2376_K

Plasmid

#146808

Purpose

Mammalian Expression of HsNot1iso1_1595-2376

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1595-2376 (CNOT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2

Plasmid

#196534

Purpose

Expression of GST-Eps8L2-FL

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5mycGW-Eps8L2_529-715

Plasmid

#196543

Purpose

Expression of myc-Eps8L2_529-715

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_529-715 (EPS8L2 Human)

Tags

myc

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b I-SceI (Ctermhis) K223I

Plasmid

#195615

Purpose

Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity

Depositor

Frederick Gimble

Article

Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

Insert

I-SceI

Expression

Bacterial

Mutation

C2694G, G2345C, G2351C, A3365T, A3366T

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET PI-SceI C-His K301A

Plasmid

#195611

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutation

Depositor

Frederick Gimble

Article

He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

Insert

PI-SceI

Expression

Bacterial

Mutation

A822G, A823C, A824T

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-DmNot3_677-844_P

Plasmid

#147185

Purpose

Bacterial Expression of DmNot3_677-844

Depositor

Elisa Izaurralde

Insert

DmNot3_677-844 (Not3 Fly)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-DmNot3_677-844_P

Plasmid

#147190

Purpose

Bacterial Expression of DmNot3_677-844

Depositor

Elisa Izaurralde

Insert

DmNot3_677-844 (Not3 Fly)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCavin1a in pT3TS-Dest

Plasmid

#194294

Purpose

In vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAI

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1a (cavin1a Zebrafish)

Use

In vitro transcription of mrna

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Notch1 #2 GFP

Plasmid

#106951

Purpose

Lentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

gRNA targeting Notch1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Notch1 #3 GFP

Plasmid

#106952

Purpose

Lentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

gRNA targeting Notch1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2114

Plasmid

#175961

Purpose

For expression of LetA-[ΔRing6 LetB] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Tags

8xHis 2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

Δ634-746

Promoter

araBAD

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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2xNLS-NLP-cMyc AspCas12a

Plasmid

#182120

Purpose

pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

2xNLS-NLP-cMyc AspCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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KBB556

Plasmid

#185108

Purpose

1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with S158A, F159A, F160A

Depositor

Ken Belanger

Article

Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

Insert

POM152

Tags

GFP

Expression

Yeast

Mutation

1st 200 amino acids (N-term + transmembrane domai…

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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KBB553

Plasmid

#185107

Purpose

1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with Y180L, Q182L

Depositor

Ken Belanger

Article

Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

Insert

POM152

Tags

GFP

Expression

Yeast

Mutation

1st 200 amino acids (N-term + transmembrane domai…

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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KBB438

Plasmid

#185092

Purpose

GAL1::Rfa1[1-337, 344-621]-GFP with NLS removed

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA1

Expression

Yeast

Mutation

Rfa1 with amino acids 338-343 deleted

Promoter

GAL1

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB372

Plasmid

#185084

Purpose

GAL::Rfa1[330-621]-GFP under control of GAL1 promoter

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA1

Tags

GFP

Expression

Yeast

Mutation

Rfa1 amino acids 330-621

Promoter

GAL1

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF1-L1-dSpRY

Plasmid

#177681

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dCas9-SpRY

Use

CRISPR; Gateway compatible foki-dcas9-spry entry …

Tags

NLS

Expression

Plant

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF1-L2-dSpRY

Plasmid

#177682

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dCas9-SpRY

Use

CRISPR; Gateway compatible foki-dcas9-spry entry …

Tags

NLS

Expression

Plant

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot7-M141R_R

Plasmid

#147379

Purpose

Mammalian Expression of HsNot7-M141R

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7-M141R (CNOT7 Human)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-HsNot7_9-260_R

Plasmid

#147369

Purpose

Bacterial Expression of HsNot7_9-260

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7_9-260 (CNOT7 Human)

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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