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We narrowed to 19,160 results for: Tec

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Showing: 9601 - 9620 of 19160 results

  1. AA315

    Plasmid
    #215951
    Purpose
    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control lethal
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    rev{U6_v1; DR_v0 [EnAs]; sgPLK1_v1 [EnAs]; DR_v1 [EnAs]; sgPLK1_v2 [EnAs]}
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215951-plasmid-map-sequence-id-431482

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  2. AA074

    Plasmid
    #215933
    Purpose
    Fragmid fragment: (guide cassette) Tet-regulatable guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    H1-2xTetO_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs]
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215933-plasmid-map-sequence-id-431475

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  3. AA435

    Plasmid
    #215962
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; DR_v0 [EnAs]; sgCD274_v1; DR_v1 [EnAs]; sgADGRE5_v3; DR_v2; sgCD4_v1; DR_v3; sgDPP4_v1
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215962-plasmid-map-sequence-id-431471

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  4. AA021

    Plasmid
    #215929
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs];
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215929-plasmid-map-sequence-id-431458

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  5. AA083

    Plasmid
    #215934
    Purpose
    Fragmid fragment: (guide cassette) reverse orientation; guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    rev{U6_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs]}
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215934-plasmid-map-sequence-id-431445

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  6. pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)

    Plasmid
    #208977
    Purpose
    Entry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Promoter
    human U6
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208977-plasmid-map-sequence-id-416230

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  7. Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)

    Plasmid
    #208974
    Purpose
    Recruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    B103-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208974-plasmid-map-sequence-id-415835

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  8. Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

    Plasmid
    #208971
    Purpose
    Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Phi29-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Phi29(-exo;D169A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208971-plasmid-map-sequence-id-415826

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  9. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

    Plasmid
    #208970
    Purpose
    Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Phi29(+exo)-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208970-plasmid-map-sequence-id-415821

    Enlarge

  10. B-HeFSpCas9-A695Q

    Plasmid
    #207368
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A695Q
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available Since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    207368-plasmid-map-sequence-id-410902

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  12. B-HeFSpCas9-A926Q

    Plasmid
    #207370
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A926Q
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207370-plasmid-map-sequence-id-410164

    Enlarge

  13. B-evoSpCas9-E526K

    Plasmid
    #207365
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-evoSpCas9-E526K
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    M495V, Y515N, R661Q, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207365-plasmid-map-sequence-id-410163

    Enlarge

  14. B-evoSpCas9-V495M

    Plasmid
    #207363
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-evoSpCas9-V495M
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Y515N, K526E, R661Q, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207363-plasmid-map-sequence-id-410136

    Enlarge

  15. B-HeFSpCas9-A1060R

    Plasmid
    #207372
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A1060R
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …
    Promoter
    CBh
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207372-plasmid-map-sequence-id-408921

    Enlarge

  16. B-HeFSpCas9-A1003K

    Plasmid
    #207371
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A1003K
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …
    Promoter
    CBh
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207371-plasmid-map-sequence-id-408916

    Enlarge

  17. B-HeFSpCas9-A848K

    Plasmid
    #207369
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A848K
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available Since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207369-plasmid-map-sequence-id-408749

    Enlarge

  18. B-HeFSpCas9-A661R

    Plasmid
    #207367
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A661R
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available Since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207367-plasmid-map-sequence-id-408748

    Enlarge

  19. B-evoSpCas9-Q661R

    Plasmid
    #207366
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-evoSpCas9-Q661R
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207366-plasmid-map-sequence-id-408747

    Enlarge

  20. B-evoSpCas9-N515Y

    Plasmid
    #207364
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-evoSpCas9-N515Y
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    M495V, K526E, R661Q, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207364-plasmid-map-sequence-id-408746

    Enlarge

Showing: 9601 - 9620 of 19160 results