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pCIneo-V5-SBP-MBP-HsNanos3_4-20_X

Plasmid

#147923

Purpose

Mammalian Expression of HsNanos3_4-20

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3_4-20 (NANOS3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-EGFP-HsNanos3_4-20_X

Plasmid

#147930

Purpose

Mammalian Expression of HsNanos3_4-20

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3_4-20 (NANOS3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsSmg7_1-633_U

Plasmid

#147644

Purpose

Mammalian Expression of HsSmg7_1-633

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7_1-633 (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C and A1521G co…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5-del133-155_T

Plasmid

#147553

Purpose

Mammalian Expression of HsSmg5-del133-155

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del133-155 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg7_1-633_T

Plasmid

#147557

Purpose

Mammalian Expression of HsSmg7_1-633

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7_1-633 (SMG7 Human)

Expression

Mammalian

Mutation

two silent mutation G216A, A1521G compared to the…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsNanos3_P

Plasmid

#147200

Purpose

Mammalian Expression of HsNanos3

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3 (NANOS3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNanos3_P

Plasmid

#147203

Purpose

Mammalian Expression of HsNanos3

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3 (NANOS3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBruno_N

Plasmid

#147028

Purpose

Insect Expression of DmBruno

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmBruno (aret Fly)

Expression

Insect

Mutation

three non silent mutations S88T, H203R and A449R …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBruno_N

Plasmid

#147030

Purpose

Insect Expression of DmBruno

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmBruno (aret Fly)

Expression

Insect

Mutation

three non silent mutations S88T, H203R and A449R …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-mut2_F

Plasmid

#146272

Purpose

Insect Expression of DmMe31B-mut2

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmMe31B-mut2 (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G and one non silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-IC2N-dCer3

Plasmid

#191325

Purpose

Chronically inhibit dynein by expressing the dominant negative acting N-terminal domain of the dynein intermediate chain (IC2N)

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

IC2N-dCer-FRB (Dync1i2 Mouse)

Expression

Mammalian

Mutation

aa 1-237

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos3-Y10E_X

Plasmid

#147966

Purpose

Mammalian Expression of HsNanos3-Y10E

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-Y10E (NANOS3 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos3_X

Plasmid

#147962

Purpose

Mammalian Expression of HsNanos3

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3 (NANOS3 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos3-del4-20_X

Plasmid

#147963

Purpose

Mammalian Expression of HsNanos3-del4-20

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-del4-20 (NANOS3 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos3-F4A_X

Plasmid

#147964

Purpose

Mammalian Expression of HsNanos3-F4A

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-F4A (NANOS3 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos3-W7E_X

Plasmid

#147965

Purpose

Mammalian Expression of HsNanos3-W7E

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-W7E (NANOS3 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pA-RFP-rG2

Plasmid

#188969

Purpose

IPTG inducible mCherry with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

mCherry
sgRNA: agtccatgtaatcagcgtctactagt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSLiP-G2

Plasmid

#188964

Purpose

L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

dCas9
sgRNA: gttagacgctgattacatggactagg

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-ZmIAA29 (yeast codon optimized)

Plasmid

#188441

Purpose

Zea Mays Nuclear Auxin Signaling Repressor IAA29 with yeast codon optimization

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Repressor IAA29 (yeast codon optimized)

Use

Cloning

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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mVenus-FAK(397)FRB

Plasmid

#188660

Purpose

mVenus tagged Focal Adhesion Kinase with FRB inserted at Y397

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

Focal Adhesion Kinase

Tags

mVenus

Expression

Mammalian

Mutation

Insertion of FRB at Tyrosine 397

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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