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DJ-L1 gRNA #4

Plasmid

#247544

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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DJ-L1 gRNA #3

Plasmid

#247543

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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DJ-L1 gRNA #1

Plasmid

#247542

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-PUM2

Plasmid

#245723

Purpose

Express Flag-HA-PUM2 in mammalian cells

Depositor

Joshua Mendell

Article

Thomas et al Genes Dev. 2022 May 1;36(9-10):550-565.

Insert

Flag-HA-PUM2 (PUM2 Human)

Use

Lentiviral

Tags

Flag-HA

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCAMBIA1302-Pro35S:Irp9

Plasmid

#246319

Purpose

Overexpression of a bacterial bifunctional salicylate synthase targeted to the cytosol

Depositor

Chung-Jui Tsai

Article

Xue et al Plant Cell. 2013 Jul;25(7):2714-30. doi:

Insert

Irp9

Expression

Plant

Promoter

cauliflower mosaic virus 35S

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCAMBIA1302-Pro35S:Fd-Irp9

Plasmid

#246318

Purpose

Overexpression of a bacterial bifunctional salicylate synthase, Irp9, targeted to the chloroplast

Depositor

Chung-Jui Tsai

Article

Xue et al Plant Cell. 2013 Jul;25(7):2714-30. doi:

Insert

Fd-Irp9

Expression

Plant

Promoter

cauliflower mosaic virus 35S

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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EK0574 CAG-ADAR1-p150

Plasmid

#247268

Purpose

Expresses human ADAR1-p150 from the CAG promoter

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

ADAR1-p150 (ADAR Human)

Expression

Mammalian

Mutation

WT

Promoter

CAG

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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NSK250 CMV-TO-SCN1A (exons 20, 20N, 21)

Plasmid

#247285

Purpose

Expresses human SCN1a exons 20, 20N, 21

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

SCN1a exons 20, 20N, 21 (SCN1A Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(R4A,R5A)

Plasmid

#245221

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

R4A,R5A

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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AtHKT1_pYES2

Plasmid

#244550

Purpose

High- copy episomal vector for galactose-inducible expression of AtHKT1 protein in Saccharomyces cerevisiae.

Depositor

Julian Schroeder

Article

Uozumi et al Plant Physiol. 2000 Apr;122(4):1249-59.

Insert

AtHKT1 (HKT1 Mustard Weed)

Expression

Yeast

Promoter

T7 promoter

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pZNp-2

Plasmid

#245373

Purpose

Negative control, firefly luciferase mutated

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-wt (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZ_pF-3

Plasmid

#245374

Purpose

Negative control, NanoLuciferase mutated

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-wt (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-wt

Plasmid

#245364

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-wt (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT7R-7xsfGFP11-HA-HDR-donor

Plasmid

#221818

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT7R coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT7R homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT7R-sfGFP11-HA-CRISPR-HDR-donor

Plasmid

#221819

Purpose

Donor plasmid for inserting GFP11-HA to the end of Drosophila 5-HT7R coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

GFP11-HA donor with homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT1AR-7xsfGFP11-HA-HDR-donor

Plasmid

#221832

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1AR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT1AR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT2BR-7xsfGFP11-HA-HDR-donor

Plasmid

#221833

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2BR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_2xFlag-dSERT-HDR-donor-HDR

Plasmid

#221835

Purpose

Donor plasmid for inserting 2xFLAG at the beginning of Drosophila SERT coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

2xFLAG with dSERT homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT1BR-7xsfGFP11-HA-HDR-donor

Plasmid

#221837

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT1BR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT2AR(isoformD)-7xsfGFP11-HA-HDR-donor

Plasmid

#221838

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoform D coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2AR (isoform D) homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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