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Accessory plasmid 2

Plasmid

#128571

Purpose

for cloning identified individual SPECS

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagRFP

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pBIG1d

Plasmid

#80614

Purpose

pBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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P336-FBP_11

Plasmid

#139702

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-MEG3c

Plasmid

#59422

Purpose

Expresses lncRNA MEG3 isoform c in mammalian cells.

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

MEG3c (MEG3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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RP1B-PP1alpha

Plasmid

#51768

Purpose

Expresses PP1 alpha residues 7-330

Depositor

Wolfgang Peti

Article

Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

Insert

pp1 alpha

Tags

His, TEV protease site, and Thio-6

Expression

Bacterial

Mutation

Contains PP1 alpha residues 7-330

Promoter

T7-lac

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
pPIDNB

Plasmid

#169901

Purpose

Empty dox inducible vector with piggyBac, insulators, and a DTS

Depositor

Richard Schneider

Article

Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

Type

Empty backbone

Use

Aivian expression

Expression

Mammalian

Promoter

Dox inducible

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-MEG3d

Plasmid

#59423

Purpose

Expresses lncRNA MEG3 isoform d in mammalian cells.

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

MEG3d (MEG3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pNIR-FbLAG30

Plasmid

#220740

Purpose

NIR-FbLAG30 expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG30

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-YU2gp160

Plasmid

#100927

Purpose

Mammalian expression plasmid for Env from the YU-2 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (YU2) Env

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup93-SpeI

Plasmid

#87331

Purpose

Expression of shRNA targeting at Nup93

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup93

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-CD4-sTb(C)-M13-GFP

Plasmid

#219784

Purpose

sTb(C) half of CaST for neuron expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTb(C)-M13-GFP

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

Synapsin

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup93-XhoI

Plasmid

#87330

Purpose

To express shRNA against human Nup93

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup93

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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CRY2high-mCherry

Plasmid

#104063

Purpose

Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2high-mCherry

Expression

Mammalian

Mutation

CRY2PHR E490R

Promoter

CMV

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pANCH2-Nat

Plasmid

#87249

Purpose

gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT)

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Nat

Expression

Yeast

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pUB_SM-FLuc0-GFPOPT

Plasmid

#210594

Purpose

Expresses SM(FLAG)-nonoptimal Firefly luciferase-optimal GFP

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal FLuc-Optimal GFP

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_SM-Fluc100-GFPOPT

Plasmid

#210596

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase-optimal GFP

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc-Optimal GFP

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-6-Ruby-9

Plasmid

#197740

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Ruby reporter tricistronic CDS

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV/EF1a-DIO-SCLM

Plasmid

#216759

Purpose

AAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoter

Depositor

George Augustine

Insert

SuperClomeleon

Use

AAV

Expression

Mammalian

Mutation

S30R and 11 AA deletion in the Cerulean CFP moiet…

Promoter

EF1a (human elongation factor 1 alpha)

Available Since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS10

Plasmid

#127129

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Mutation

K24R- please see depositor comments

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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IFP2.0-N1

Plasmid

#54785

Purpose

Localization: N1 Cloning Vector, Excitation: 684, Emission: 708

Depositor

Michael Davidson, Xiaokun Shu

Article

Yu et al Nat Commun. 2014 May 15;5:3626. doi: 10.

Type

Empty backbone

Tags

IFP2.0

Expression

Mammalian

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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