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pFAB3992

Plasmid

#47823

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD16 efficiency.

Depositor

Drew Endy

Publication

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD16

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFAB3997

Plasmid

#47828

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD21 efficiency.

Depositor

Drew Endy

Publication

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD21

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3913

Plasmid

#47816

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD9 efficiency.

Depositor

Drew Endy

Publication

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD9

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB803

Plasmid

#47847

Purpose

BIOFAB reporter plasmid for measuring T7 early termination efficiency

Depositor

Drew Endy

Publication

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

T7 early terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO2

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3912

Plasmid

#47815

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD8 efficiency.

Depositor

Drew Endy

Publication

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD8

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3906

Plasmid

#47809

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD2 efficiency.

Depositor

Drew Endy

Publication

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD2

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-XRCC6BP1

Plasmid

#23675

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

XRCC6BP1 (XRCC6BP1 Human)

Use

Gateway Cloning

Mutation

A4V

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-SH3BP5L

Plasmid

#23566

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SH3BP5L (SH3BP5L Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pIS1-miR-126 site

Plasmid

#25026

Depositor

Bob Weinberg

Publication

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

synthetic miR-126 site

Use

Luciferase

Expression

Mammalian

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pEF 4r-GUS-3r

Plasmid

#20097

Depositor

Antonio Richart

Insert

GUS (gus )

Use

Synthetic Biology; Plant gateway vector

Available since

March 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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YIp LYS2-URA3

Plasmid

#122911

Purpose

LYS2-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

July 31, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx3_SpRY (ABE8e_M151E_nCas9_SpRY)

Plasmid

#251546

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the M151E mutation in the deaminase domain

Depositor

George Church

Publication

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_M151E-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx3 M151E

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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CAG::TdTomato

Plasmid

#140504

Purpose

Encodes TdTomato driven by the CAG promoter

Depositor

Mark Emerson

Publication

Schick et al Sci Rep. 2019 Jun 27;9(1):9358. doi: 10.

Insert

CAG promoter

Use

Chicken expression

Expression

Mammalian

Available since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_C-StrepIII

Plasmid

#231300

Purpose

For transiently expressing fusion proteins with C terminal StrepIII tags in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

StrepIII

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMG005_mNeonGreen_P2A

Plasmid

#237215

Purpose

Fluorescent reporter codon optimized for Dictyostelid species

Depositor

Jordi van Gestel

Insert

mNeonGreen-P2A

Use

Cloning

Tags

3xFLAG-tag, HA-tag, SV40-NLS, and nuleoplasmin NL…

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pMG008_mCherry_P2A

Plasmid

#237216

Purpose

Fluorescent reporter codon optimized for Dictyostelid species

Depositor

Jordi van Gestel

Insert

mCherry-P2A

Use

Cloning

Tags

3xFLAG-tag, HA-tag, SV40-NLS, and nuleoplasmin NL…

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAL004

Plasmid

#233863

Purpose

MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.minBG-EGFP-W3SL_2x-U6-sasgAi14

Plasmid

#231365

Purpose

minBG-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.Ple155-CI-EGFP-W3SL_2x-U6-sasgAi14

Plasmid

#231366

Purpose

Ple155-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pNK7187

Plasmid

#219761

Purpose

MoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Publication

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PpASCL

Expression

Plant

Promoter

p35s_0.4kb - 5'UTR TMV omega

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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