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pCMV_Anc689-NG-nCas9

Plasmid

#163527

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

Anc689-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-CreERT2 sgAdam10 v3

Plasmid

#158047

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Adam10. NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Adam10 sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti PA-mCit

Plasmid

#113451

Purpose

Lentiviral ProteinA-mCitrine expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine

Use

Lentiviral

Expression

Mammalian

Promoter

hUBbC

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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eTd-CBEa

Plasmid

#196603

Purpose

Mammalian expression of eTd-CBEa

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L/P29A)-nSpCas9-bpNLS-P2A-UGI-bpNLS

Tags

6*His

Expression

Mammalian

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ecDHFR(DD)-MS2CP-VPg(FCV)

Plasmid

#167313

Purpose

Template pDNA to prepare DD-CaVT mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

DD-CaVT

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-MED18

Plasmid

#15419

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED18 (MED18 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pLKO.1 p18 shRNA

Plasmid

#26631

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

p18 shRNA (LAMTOR1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pBW2209_pCAG-PV1-iCre-N229-L1-GID1-NLS-BGHpA

Plasmid

#108723

Purpose

Expresses N-terminus to aa229 of Cre recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa229

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2229_pCAG-PV1-GAI-L1-iCre-230C-NLS-BGHpA

Plasmid

#108724

Purpose

Expresses aa230 to C-terminus of Cre recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa230 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MED22

Plasmid

#15423

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED22 (MED22 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-dCas12a-A3A

Plasmid

#193638

Purpose

Expression of base editor dCas12a-A3A in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mA3-BE3

Plasmid

#113419

Purpose

Expresses mA3-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

mA3-BE3 (Apobec3 S. pyogenes and Bacteriophage PBS2, Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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DRH335: FCK-Optopatch1

Plasmid

#51695

Purpose

Coexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch1

Use

Lentiviral

Expression

Mammalian

Promoter

CaMKIIa

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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proE-cad178-Luc-mEbox

Plasmid

#42082

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

E-cadherin (CDH1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

contains E-cadherin promoter region from -178 to …

Promoter

E-cadherin

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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p59sheLL

Plasmid

#40628

Depositor

Simon Beddows

Article

Draper et al Vaccine. 2011 Nov 3;29(47):8585-90. Epub

Inserts

HPV59 L1
HPV59 L2

Expression

Mammalian

Mutation

Codon optimized for mammalian expression

Promoter

CMV and Polio IRES

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pBa-FRB-3myc-Rab7

Plasmid

#64210

Purpose

Rab7 with N-terminal FRB and triple myc tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Rab7 (Rab7 Mouse)

Tags

FRB and triple myc tag (3myc)

Expression

Mammalian

Promoter

chicken Beta Actin

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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phROSA26-Tet-HA-JLP_WT

Plasmid

#208770

Purpose

Tetracycline inducible expression vector for HA-tagged wild-type JLP protein (HA-JLP_WT), used as a donor plasmid for HA-JLP_WT knock-in at the human ROSA26 locus

Depositor

Katsuji Yoshioka

Article

Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

Insert

JLP (SPAG9 Human)

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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ACTN3-pEGFP

Plasmid

#52670

Purpose

Overexpression of GFP-tagged α-actinin-3

Depositor

Johannes Hell

Article

Hall et al Neuron. 2013 May 8;78(3):483-97. doi: 10

Insert

ACTN3 (ACTN3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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pSLQ7556 pHR (hU6-crCD46-EFS-PuroR-WPRE)

Plasmid

#214880

Purpose

Lentiviral vector encoding RfxCas13d targeting CD46 guide

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crCD46-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 tTA UQCRC2

Plasmid

#177978

Purpose

All-in-one dox-repressible expression of cDNA

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

UQCRC2 (UQCRC2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Silent point mutations to UQCRC2 sgRNA target

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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