We narrowed to 14,608 results for: pET

1. bdSUMO-pHlip-GFP-HiBit

   Plasmid

   #171016

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-pHlip-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Prb(Asp83Asn)

   Plasmid

   #24833

   Depositor

   David Baker

   Article

   Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

   Insert

   Prb(Asp83Asn)
   

   Use

   Tags

   Avi and His

   Expression

   Bacterial

   Mutation

   Asp83Asn

   Promoter

   Available since

   April 14, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pMSP1D1T1

   Plasmid

   #97327

   Purpose

   Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

   Depositor

   Michael Marty

   Article

   Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

   Insert

   MSP1D1T1
   

   Use

   Tags

   6xHis - TEV

   Expression

   Bacterial

   Mutation

   Addition of T residue after T25

   Promoter

   T7

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSP1D1T2

   Plasmid

   #97328

   Purpose

   Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

   Depositor

   Michael Marty

   Article

   Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

   Insert

   MSP1D1T2
   

   Use

   Tags

   6xHis - TEV

   Expression

   Bacterial

   Mutation

   Addition of two T residues after T25

   Promoter

   T7

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLz74_Rubisco_CsoS2_Npeptide_fusion

   Plasmid

   #140840

   Purpose

   Same as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallization

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   Form IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS
   

   Use

   Tags

   CsoS2 N-peptide consensus to CbbL C-terminus and …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p300 KAT LIC 1M

   Plasmid

   #233587

   Purpose

   Bacterial expression of p300 KAT enzyme

   Depositor

   Scott Showalter

   Article

   Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

   Insert

   p300 KAT (EP300 Human)
   

   Use

   Tags

   6xHis MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAM59

   Plasmid

   #227655

   Purpose

   pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, Δ78–81)

   Depositor

   Andreas Martin

   Article

   Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

   Inserts

   Rpn11 (RPN11 Budding Yeast)
   Rpn8 (RPN8 Budding Yeast)
   

   Use

   Tags

   H6-PreScission and PreScission-Strep

   Expression

   Bacterial

   Mutation

   1-179 and amino acids 2-239, Δ78–81

   Promoter

   T7

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. VF-Sensor

   Plasmid

   #208927

   Purpose

   in vitro RSK-Sensor for luminescent detection of RSK-activity

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Insert

   SOS1 (SOS1 Human)
   

   Use

   Tags

   HISx6, MBP, and Smbit

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PDE4B-Sensor

   Plasmid

   #208929

   Purpose

   in vitro JNK-Sensor for luminescent detection of JNK-activity

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Insert

   PDE4B docking sequence+Phospho site and Smbit (PDE4B Human)
   

   Use

   Tags

   HISx6, MBP, and Smbit

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSC-cUnaG

    Plasmid

    #207631

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-cUnaG
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    T7

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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