We narrowed to 10,059 results for: ADA

1. pαH-S-RRAR

   Plasmid

   #164569

   Purpose

   SARS-CoV-2 Spike protein with Furin site restored (S-RRAR variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-RRAR variant)
   

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5 FRT/TO/STRP 3x FLAG cyclin B1 L45A (1391)

   Plasmid

   #61841

   Purpose

   mammalian expression of cyclin B1 non-degradable L45A mutant

   Depositor

   Jonathon Pines

   Article

   Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

   Insert

   cyclin B1 (CCNB1 Human)
   

   Tags

   2xSTREP and 3xFLAG

   Expression

   Mammalian

   Mutation

   L45A

   Promoter

   CMV

   Available Since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

3. ABE8.17-d

   Plasmid

   #136299

   Purpose

   expresses ABE8.17-d in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.20-d
   

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pblic-neo TRX1

   Plasmid

   #110919

   Purpose

   To overexpress Thioredoxin-1

   Depositor

   Priyamvada Rai

   Article

   Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

   Insert

   TRX1 (TXN Human)
   

   Use

   Retroviral

   Promoter

   unknown

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. HTRA1_PDZ_1

   Plasmid

   #103912

   Purpose

   Protein expression and purification of HTRA1 PDZ domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   HTRA1 (HTRA1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC18T-mini-Tn7T-Gm-Pc-mKate2

   Plasmid

   #199249

   Purpose

   Fluorescent labelling of bacterial cells with mKate2

   Depositor

   Uwe Mamat

   Article

   Mamat et al Appl Environ Microbiol. 2023 May 17:e003

   Insert

   Codon-optimized mKate2 gene
   

   Expression

   Bacterial

   Mutation

   Codon optimization for Xanthomonadaceae

   Promoter

   Pc promoter from class III integron of Delftia a…

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. hGATA-1 K137R

   Plasmid

   #118353

   Purpose

   This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hGATA-1 (GATA1 Human)
   

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K137R

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PDZb-beta2(616-951)-mCherry

   Plasmid

   #100736

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2B1 (AP2B1 Human)
   

   Tags

   PDZb and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYFP-NGAT(A193T,N194Y)

   Plasmid

   #11388

   Depositor

   Joel Swanson

   Article

   Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

   Insert

   golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Changed Alanine 193 to Threonine, and Asparagine …

   Available Since

   March 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-BE3RA-P2A-Puro

    Plasmid

    #110839

    Purpose

    Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Mutation

    D10A

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti ABE7.10-N-AIDmax

    Plasmid

    #157949

    Purpose

    Lentiviral expression of ABE7.10-N-AIDmax

    Depositor

    Dali Li

    Article

    Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Lenti ABE7.10-N-AIDmax
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. HT116_pAAV_hSyn-DiO-SomQuasAr6b_EGFP

    Plasmid

    #190879

    Purpose

    Cre-on expression of soma-targeted QuasAr6b under an neuronal promoter

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    soma-targeted QuasAr6b
    

    Use

    AAV

    Tags

    EGFP

    Promoter

    hSyn

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ABE8.8-d

    Plasmid

    #136295

    Purpose

    expresses ABE8.8-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.8-d
    

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAT15546_nABE8e-SuperFi-Cas9

    Plasmid

    #184376

    Purpose

    Mammalian expression of SuperFi-Cas9 ABE8e base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    nABE8e-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-beta2(616-951)

    Plasmid

    #100743

    Purpose

    Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Long isoform

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAT15544_nABE-SuperFi-Cas9

    Plasmid

    #184374

    Purpose

    Mammalian expression of SuperFi-Cas9 ABE7 base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    nABE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    FLAG, SV40 NLS, and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. N-His-GvpC-ssrA

    Plasmid

    #153297

    Purpose

    Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
    

    Tags

    His-tag and ssrA tag

    Expression

    Bacterial

    Mutation

    1) N-terminal 6xHis tag flanked by 1x glycine res…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMGF157

    Plasmid

    #96972

    Purpose

    ish1-mCherry insertion cassette with Hygro resistance gene

    Depositor

    Wesley Sundquist, Adam Frost

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + hygro…

    Promoter

    N/A

    Available Since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. ABE8.13-d

    Plasmid

    #136297

    Purpose

    expresses ABE8.13-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.13-d
    

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-BE3-P2A-Puro

    Plasmid

    #110838

    Purpose

    Lentiviral vector for constitutive expression of BE3 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Mutation

    D10A

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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